Home LiteratureArticle Details
PMID: 3117804 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An investigation of microtubule organization and functions in living Drosophila embryos by injection of a fluorescently labeled antibody against tyrosinated alpha-tubulin.

The Journal of cell biology ·Vol. 105 ·No. 4 ·1987-10-00 ·Pages 1721-30

Warn RM, Flegg L, Warn A

Abstract

Rhodamine-labeled monoclonal antibodies, which react with tyrosinated alpha-tubulin (clone YL 1/2; Kilmartin, J. V., B. Wright, and C. Milstein, 1982, J. Cell Biol., 93:576-582) and label microtubules in vivo (Wehland, J., M. C. Willingham, and I. Sandoval, 1983, J. Cell Biol., 97:1467-1475) were microinjected into syncytial stage Drosophila embryos. At 1 mg/ml antibody concentration, the microtubule arrays of the surface caps became labeled by YL 1/2 but normal development was found to continue. The results are compared with the data from fixed material particularly with regard to interphase microtubules, centrosome separation, and spindle and midbody formation. At 5 mg/ml antibody concentration the microtubules took up larger quantities of antibodies and clumped around the nuclei. Nuclei with clumped microtubules lost their position in the surface layer and moved into the interior. As a result, the F-actin cap meshwork associated with such nuclei either failed to form or subsided. It is concluded that microtubule activity is required to maintain the nuclei in the surface layer and organize the F-actin meshwork of the caps.

MeSH Terms
Actin Cytoskeleton/physiology,ultrastructure Animals Antibodies, Monoclonal/administration & dosage Cell Compartmentation Cell Cycle Cytoskeleton/ultrastructure Dose-Response Relationship, Immunologic Drosophila melanogaster/embryology,ultrastructure Microinjections Microtubules/physiology Mitosis Spindle Apparatus/ultrastructure Tubulin/physiology Tyrosine
Chemicals
Antibodies, Monoclonal Tubulin Tyrosine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Warn R M
School of Biological Sciences, University of East Anglia, Norwich, United Kingdom.
Flegg L
Warn A
References (38)
38 references, click to expand
  1. Developmental analysis of the grandchildless (gs(1)N26) mutation in Drosophila melanogaster: abnormal cleavage patterns and defects in pole cell formation.
    Dev Biol. 1984 May;103(1):182-9 PMID: 6425095
  2. Distribution of F-actin during cleavage of the Drosophila syncytial blastoderm.
    J Cell Biol. 1984 Jan;98(1):156-62 PMID: 6423648
  3. Regulation of Drosophila alpha- and beta-tubulin genes during development.
    Dev Biol. 1984 Jul;104(1):187-98 PMID: 6329853
  4. Amino acid sequence requirements in the epitope recognized by the alpha-tubulin-specific rat monoclonal antibody YL 1/2.
    EMBO J. 1984 Jun;3(6):1295-300 PMID: 6204858
  5. Analysis of the role of microfilaments and microtubules in acquisition of bipolarity and elongation of fibroblasts in hydrated collagen gels.
    J Cell Biol. 1984 Aug;99(2):536-49 PMID: 6146628
  6. Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: video image analysis of fluorescence redistribution after photobleaching.
    J Cell Biol. 1984 Dec;99(6):2157-64 PMID: 6501417
  7. Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching.
    J Cell Biol. 1984 Dec;99(6):2165-74 PMID: 6501418
  8. Tubulin dynamics in cultured mammalian cells.
    J Cell Biol. 1984 Dec;99(6):2175-86 PMID: 6501419
  9. Three distinct distributions of F-actin occur during the divisions of polar surface caps to produce pole cells in Drosophila embryos.
    J Cell Biol. 1985 Apr;100(4):1010-5 PMID: 3980576
  10. Distribution of fluorescently labeled tubulin injected into sand dollar eggs from fertilization through cleavage.
    J Cell Biol. 1985 Apr;100(4):1262-72 PMID: 3920225
  11. Polymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes.
    J Cell Biol. 1985 May;100(5):1682-9 PMID: 3886672
  12. The small subunit of ribonucleotide reductase is encoded by one of the most abundant translationally regulated maternal RNAs in clam and sea urchin eggs.
    J Cell Biol. 1985 Jun;100(6):1968-76 PMID: 2987274
  13. Cell cycle control by the nucleo-cytoplasmic ratio in early Drosophila development.
    Cell. 1986 Jan 31;44(2):365-72 PMID: 3080248
  14. Microtubule arrays present during the syncytial and cellular blastoderm stages of the early Drosophila embryo.
    Exp Cell Res. 1986 Mar;163(1):201-10 PMID: 3943561
  15. Microtubule dynamics in interphase cells.
    J Cell Biol. 1986 Mar;102(3):1020-31 PMID: 3512576
  16. Organization of the cytoskeleton in early Drosophila embryos.
    J Cell Biol. 1986 Apr;102(4):1494-509 PMID: 3514634
  17. Sites of microtubule assembly and disassembly in the mitotic spindle.
    Cell. 1986 May 23;45(4):515-27 PMID: 3708686
  18. The dissociation of nuclear and centrosomal division in gnu, a mutation causing giant nuclei in Drosophila.
    Cell. 1986 Aug 1;46(3):457-68 PMID: 3089628
  19. Tissue-specific and constitutive alpha-tubulin genes of Drosophila melanogaster code for structurally distinct proteins.
    Proc Natl Acad Sci U S A. 1986 Nov;83(22):8477-81 PMID: 3095837
  20. The cytoskeleton of the early Drosophila embryo.
    J Cell Sci Suppl. 1986;5:311-28 PMID: 3308915
  21. Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin.
    Science. 1975 Mar 14;187(4180):948-50 PMID: 1096300
  22. Specific visualization of tubulin-containing structures in tissue culture cells by immunofluorescence. Cytoplasmic microtubules, vinblastine-induced paracrystals, and mitotic figures.
    Exp Cell Res. 1975 Oct 1;95(1):111-20 PMID: 172344
  23. Simultaneous localization of myosin and tubulin in human tissue culture cells by double antibody staining.
    J Cell Biol. 1978 Apr;77(1):182-95 PMID: 350890
  24. Distribution of tubulin-containing structures in the egg of the sea urchin Strongylocentrotus purpuratus from fertilization through first cleavage.
    J Cell Biol. 1980 Mar;84(3):668-79 PMID: 6987246
  25. Fluorescently labelled molecules as probes of the structure and function of living cells.
    Nature. 1980 Apr 3;284(5755):405-10 PMID: 6987537
  26. Direct visualization of fluorescein-labeled microtubules in vitro and in microinjected fibroblasts.
    J Cell Biol. 1981 Jan;88(1):234-40 PMID: 7193677
  27. Cytochalasin separates microtubule disassembly from loss of asymmetric morphology.
    J Cell Biol. 1981 Apr;89(1):157-61 PMID: 7014572
  28. Site and timing of synthesis of tubulin and other proteins during oogenesis in Drosophila melanogaster.
    Dev Biol. 1981 Sep;86(2):272-84 PMID: 6793425
  29. Observations by a novel method of surface changes during the syncytial blastoderm stage of the Drosophila embryo.
    Dev Biol. 1982 Feb;89(2):540-8 PMID: 6799345
  30. Developmental regulation of Drosophila alpha-tubulin genes.
    Cell. 1982 May;29(1):91-8 PMID: 6179633
  31. Microinjection of fluorescently labeled proteins into living cells with emphasis on cytoskeletal proteins.
    Int Rev Cytol. 1982;75:209-14 PMID: 6809685
  32. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.
    J Cell Biol. 1982 Jun;93(3):576-82 PMID: 6811596
  33. Organization and expression of alpha-tubulin genes in Drosophila melanogaster. One member of the alpha-tubulin multigene family is transcribed in both oogenesis and later embryonic development.
    J Mol Biol. 1982 Apr 15;156(3):449-66 PMID: 6811752
  34. Studies of nuclear and cytoplasmic behaviour during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis.
    J Cell Sci. 1983 May;61:31-70 PMID: 6411748
  35. Microinjection of fluorescent tubulin into dividing sea urchin cells.
    J Cell Biol. 1983 Oct;97(4):1249-54 PMID: 6684663
  36. A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo.
    J Cell Biol. 1983 Nov;97(5 Pt 1):1467-75 PMID: 6415068
  37. A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. II. Effects on cell movement, organization of microtubules, and intermediate filaments, and arrangement of Golgi elements.
    J Cell Biol. 1983 Nov;97(5 Pt 1):1476-90 PMID: 6685128
  38. Dynamics of the nuclear envelope and of nuclear pore complexes during mitosis in the Drosophila embryo.
    Eur J Cell Biol. 1984 May;34(1):179-89 PMID: 6428889
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.105.4.1721
Published
1987-10-00
Pages
1721-30
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114672
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.105.4.1721 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2