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PMID: 3122213 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of immunoglobulin transcription rates and mRNA processing in proliferating normal B lymphocytes by activators of protein kinase C.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 9135-9

Högbom E, Mårtensson IL, Leanderson T

Abstract

Immunoglobulin gene expression in normal splenic B lymphocytes stimulated with lipopolysaccharide was selectively down-regulated by anti-IgM antibodies and a protein-kinase C-activating phorbol ester, phorbol 12,13-dibutyrate. This control was concomitant with a decreased rate of transcription of the IgM gene while "polymerase pausing" was induced in the IgD gene. The suppression was resistant to treatment with cycloheximide, indicating that it was not caused by a labile repressor protein. The down-regulation of immunoglobulin gene expression affected only the secretory form of immunoglobulin, while the mRNA levels for the membrane-bound form of immunoglobulin remained unaltered. We conclude that the mechanisms controlling immunoglobulin gene expression in untransformed B lymphocytes differ from those operating in tumors derived from the same cell lineage.

MeSH Terms
Animals Antibodies, Anti-Idiotypic/immunology B-Lymphocytes/physiology Cell Differentiation/drug effects Cell Division Cycloheximide/pharmacology Gene Expression Regulation/drug effects Genes, Immunoglobulin Immunoglobulin M/immunology Immunoglobulin mu-Chains/genetics,metabolism Lipopolysaccharides/pharmacology Mice Phorbol Esters/pharmacology Protein Kinase C/physiology RNA Processing, Post-Transcriptional RNA, Messenger/genetics Transcription, Genetic/drug effects
Chemicals
Antibodies, Anti-Idiotypic Immunoglobulin M Immunoglobulin mu-Chains Lipopolysaccharides Phorbol Esters RNA, Messenger Cycloheximide Protein Kinase C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Högbom E
Department of Immunology, University of Uppsala Biomedical Center, Sweden.
Mårtensson I L
Leanderson T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.9135
Published
1987-12-00
Pages
9135-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299707
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.24.9135 PMC Full Text View journal details
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