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PMID: 3125519 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization and use of the Drosophila metallothionein promoter in cultured Drosophila melanogaster cells.

Nucleic acids research ·Vol. 16 ·No. 3 ·1988-02-11 ·Pages 1043-61

Bunch TA, Grinblat Y, Goldstein LS

Abstract

The promoter from the metallothionein gene may be a useful conditional promoter for the construction of chimeric genes to be expressed in Drosophila cells in culture. To explore this possibility the responses of the endogenous metallothionein gene and an in vitro constructed chimeric gene containing the metallothionein promoter were examined. Copper and cadmium, when added to the growth medium of Drosophila Schneider's line 2 cells, can produce a 30-100 fold induction of metallothionein mRNA levels. The level of induction depends on the amount of copper or cadmium added to the medium and these mRNA levels remain high for at least four days. Copper is less toxic than cadmium and does not induce a typical heat-shock response in the cells. Finally, a chimeric gene containing the metallothionein promoter shows a similar induction when transformed into the cells.

MeSH Terms
Animals Cadmium/pharmacology Cells, Cultured Cloning, Molecular Copper/pharmacology DNA, Recombinant Drosophila melanogaster/genetics Gene Expression Regulation/drug effects Genetic Vectors Heat-Shock Proteins/biosynthesis,genetics Metallothionein/genetics Promoter Regions, Genetic/drug effects RNA, Messenger/biosynthesis
Chemicals
DNA, Recombinant Heat-Shock Proteins RNA, Messenger Cadmium Copper Metallothionein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bunch T A
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
Grinblat Y
Goldstein L S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.3.1043
Published
1988-02-11
Pages
1043-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334736
Subset
IM
Grants
NIGMS NIH HHS · GM29301 · United States
NIGMS NIH HHS · GM35252 · United States
External Links
PubMed source DOI: 10.1093/nar/16.3.1043 PMC Full Text View journal details
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