Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II.

The EMBO journal ·Vol. 7 ·No. 2 ·1988-02-00 ·Pages 325-32

Pakrasi HB, Williams JG, Arntzen CJ

Abstract

The genes encoding the two subunits (alpha and beta) of the cytochrome b559 (cyt b559) protein, psbE and psbF, were cloned from the unicellular, transformable cyanobacterium, Synechocystis 6803. Cyt b559, an intrinsic membrane protein, is a component of photosystem II, a membrane-protein complex that catalyzes photosynthetic oxygen evolution. However, the role of cyt b559 in photosynthetic electron transport is yet to be determined. A high degree of homology was found between the cyanobacterial and green plant chloroplastidic psbE and psbE genes and in the amino acid sequences of their corresponding protein products. Cartridge mutagenesis techniques were used to generate a deletion mutant of Synechocystis 6803 in which the psbE and psbF genes were replaced by a kanamycin-resistance gene cartridge. Physiological analyses indicated that the PSII complexes of the mutant were inactivated. We conclude that cyt b559 is an essential component of PSII.

MeSH Terms

Amino Acid Sequence Base Sequence Chlorophyll/genetics,metabolism Cyanobacteria/genetics,growth & development,metabolism Cytochrome b Group/genetics,metabolism Electron Transport Genes Kinetics Light-Harvesting Protein Complexes Macromolecular Substances Molecular Sequence Data Mutation Photosynthesis Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins/genetics,metabolism

Chemicals

Cytochrome b Group Light-Harvesting Protein Complexes Macromolecular Substances Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins Chlorophyll cytochrome b559

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pakrasi H B

Central Research and Development Department, E.I. duPont de Nemours and Co., Wilmington, DE 19898.

Williams J G

Arntzen C J

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1988-02-00

Pages

325-32

Language

English

Region

England

NLM ID

8208664

PMCID

PMC454321

Subset

Databases

GENBANK

X06988

External Links

PubMed source PMC Full Text View journal details

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Targeted mutagenesis of the psbE and psbF genes blocks photosynthetic electron transport: evidence for a functional role of cytochrome b559 in photosystem II.

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