Home LiteratureArticle Details

PMID: 3130545 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The regulation of the fumarase (citG) gene of Bacillus subtilis 168.

Molecular & general genetics : MGG ·Vol. 211 ·No. 3 ·1988-03-00 ·Pages 465-71

Feavers IM, Price V, Moir A

Abstract

The level of fumarase activity in Bacillus subtilis depends on the nutritional environment; in rich medium low vegetative levels increase towards the end of the exponential phase, whereas in minimal glucose medium levels are relatively high throughout growth. Analysis of the enzyme levels in spoO mutants has revealed that a functional spoOH gene is required for the efficient expression of fumarase in both media. This highlights a regulatory role for the spoOH gene product not only in control of postexponentially expressed genes, but also during vegetative growth in defined medium. S1 transcript mapping reveals three transcriptional startpoints for the fumarase structural gene (citG) in B. subtilis. The upstream promoter region P1, which appears to contain two transcriptional startpoints, is functional in both Escherichia coli and B. subtilis. Promoter P2, which is located closer to the structural gene, is only functional in B. subtilis. Transcription from this promoter is strictly dependent on a functional spoOH gene; this gene has recently been shown to encode a minor sigma factor.

MeSH Terms

Amino Acid Sequence Bacillus subtilis/enzymology,genetics Base Sequence Fumarate Hydratase/genetics,metabolism Genes Genes, Bacterial Genes, Regulator Genotype Kinetics Molecular Sequence Data Mutation Promoter Regions, Genetic Transcription, Genetic

Chemicals

Fumarate Hydratase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Feavers I M

Department of Microbiology, University of Sheffield, UK.

Price V

Moir A

References (28)

28 references, click to expand

Transcriptional regulation of the spo0F gene of Bacillus subtilis.
J Bacteriol. 1986 Nov;168(2):870-7 PMID: 2430944
Effect of different nutritional conditions on the synthesis of tricarboxylic acid cycle enzymes.
J Bacteriol. 1967 Jun;93(6):1777-87 PMID: 4960893
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.
J Bacteriol. 1961 May;81(5):741-6 PMID: 16561900
Genetics analysis of spore germination mutants of Bacillus subtilis 168: the correlation of phenotype with map location.
J Gen Microbiol. 1979 Mar;111(1):165-80 PMID: 110906
Genetic studies of a secondary RNA polymerase sigma factor in Bacillus subtilis.
J Bacteriol. 1987 Aug;169(8):3464-9 PMID: 3112122
Positive regulatory gene for temperature-controlled proteins in Escherichia coli.
Biochem Biophys Res Commun. 1981 May 29;100(2):894-900 PMID: 7023474
Cloning of a developmentally regulated element from alkalophilic Bacillus subtilis DNA.
J Bacteriol. 1985 Jan;161(1):158-63 PMID: 2981799
Complete nucleotide sequence of the fumarase gene (citG) of Bacillus subtilis 168.
Nucleic Acids Res. 1985 Jan 11;13(1):131-40 PMID: 3923430
Relationship between aconitase gene expression and sporulation in Bacillus subtilis.
J Bacteriol. 1987 Jul;169(7):3068-75 PMID: 3110134
The nucleotide sequence of a spore germination gene (gerA) of Bacillus subtilis 168.
Gene. 1985;38(1-3):95-102 PMID: 2998952
Chromosomal location of pleiotropic negative sporulation mutations in Bacillus subtilis.
Genetics. 1973 Feb;73(2):215-28 PMID: 4633157
Identification of the promoter of the Bacillus subtilis sdh operon.
J Bacteriol. 1987 Jul;169(7):3232-6 PMID: 3036777
Regulation of the dicarboxylic acid part of the citric acid cycle in Bacillus subtilis.
J Bacteriol. 1975 Apr;122(1):224-34 PMID: 804468
The use of thin acrylamide gels for DNA sequencing.
FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
Regulation of spo0H, an early sporulation gene in bacilli.
J Bacteriol. 1987 Mar;169(3):1182-91 PMID: 3102457
Analysis of sporulation mutants. II. Mutants blocked in the citric acid cycle.
J Bacteriol. 1968 Apr;95(4):1431-8 PMID: 4967197
Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation.
Cell. 1983 Nov;35(1):275-83 PMID: 6414720
Bacillus subtilis spo0H gene.
J Bacteriol. 1984 Feb;157(2):405-12 PMID: 6420389
Genetic aspects of bacterial endospore formation.
Bacteriol Rev. 1976 Dec;40(4):908-62 PMID: 12736
The isolation of lambda transducing phages carrying the citG and gerA genes of Bacillus subtilis.
J Gen Microbiol. 1983 Feb;129(2):303-10 PMID: 6302200
The effect of spo0 mutations on the expression of spo0A- and spo0F-lacZ fusions.
Mol Gen Genet. 1986 Oct;205(1):28-33 PMID: 3099127
High-sensitivity S1 mapping with single-stranded [32P]DNA probes synthesized from bacteriophage M13mp templates.
Gene. 1984 Oct;30(1-3):63-8 PMID: 6096224
New RNA polymerase sigma factor under spo0 control in Bacillus subtilis.
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9438-42 PMID: 3099284
Citric acid cycle: gene-enzyme relationships in Bacillus subtilis.
J Bacteriol. 1970 Nov;104(2):826-33 PMID: 4992371
Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene.
Nature. 1983 Apr 28;302(5911):800-4 PMID: 6405278
Characterization of the fumarase gene of Bacillus subtilis 168 cloned and expressed in Escherichia coli K12.
J Gen Microbiol. 1984 Nov;130(11):3009-17 PMID: 6098632
Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase.
Cell. 1983 Nov;35(1):285-93 PMID: 6313226
Close contacts between sigma 37-RNA polymerase and a Bacillus subtilis chromosomal promoter.
J Mol Biol. 1982 Dec 15;162(3):709-13 PMID: 6820071

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00425702

Published

1988-03-00

Pages

465-71

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00425702

Analysis Services

The regulation of the fumarase (citG) gene of Bacillus subtilis 168.

Contact

WeChat Official Account