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PMID: 3133178 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A human-derived probe, p82H, hybridizes to the centromeres of gorilla, chimpanzee, and orangutan.

Chromosoma ·Vol. 96 ·No. 4 ·1988-00-00 ·Pages 270-4

Miller DA, Sharma V, Mitchell AR

Abstract

A human-derived centromeric sequence, p82H, hybridizes to DNA from gorilla, chimpanzee, pygmy chimpanzee, and orangutan. On DNA blots, multimeric ladders based on 170 or 340 bp repeat units are seen. In metaphase chromosome preparations from these species, p82H hybridizes to the centromeric region of every chromosome. p82H forms less stable hybrids with DNA from the lion-tailed macaque and does not hybridize to DNA or chromosomes of the owl monkey or the mouse.

MeSH Terms
Animals Cell Line Cells, Cultured Centromere/physiology Chromosomes/physiology Female Gorilla gorilla/genetics Haplorhini/genetics Hominidae/genetics Humans Hybrid Cells/cytology Karyotyping Leukocytes/cytology Male Mice Nucleic Acid Hybridization Pan troglodytes/genetics Pongo pygmaeus/genetics
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller D A
Department of Molecular Biology and Genetics, Wayne State University, Detroit, MI 48201.
Sharma V
Mitchell A R
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00286913
Published
1988-00-00
Pages
270-4
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · GM34959 · United States
External Links
PubMed source DOI: 10.1007/BF00286913 View journal details
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