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PMID: 3133553 Published · ppublish English Comparative Study Journal Article

fra-1: a serum-inducible, cellular immediate-early gene that encodes a fos-related antigen.

Molecular and cellular biology ·Vol. 8 ·No. 5 ·1988-05-00 ·Pages 2063-9

Cohen DR, Curran T

Abstract

A set of proteins antigenically related to the c-fos protein (Fos) are induced by serum in fibroblasts. To isolate cDNA clones of genes encoding such proteins, a lambda gt11 expression cDNA library constructed from serum-stimulated rat fibroblasts was screened with antibodies raised against a hydrophilic region (amino acids 127 to 152) of Fos. One of the positive clones identified, termed fra-1 (Fos-related antigen) was characterized. It encoded a protein that shared several regions of extensive amino acid homology with Fos (including the region that showed similarity to both the yeast GCN4 regulatory protein and the protein encoded by the jun oncogene), although its nucleotide sequence was considerably diverged from that of the c-fos gene. Only a subset of the agents and conditions that activated c-fos also induced fra-1. Induction of fra-1 expression following serum stimulation was delayed compared with that of c-fos. However, like c-fos, fra-1 was induced rapidly by serum in the presence of protein synthesis inhibitors. Thus, a family of Fos-related, inducible genes are involved in the cellular immediate-early transcriptional response to extracellular stimuli.

MeSH Terms
Amino Acid Sequence Animals Antigens/genetics Base Sequence Blood Physiological Phenomena Cell Line DNA/genetics Fibroblasts/immunology,metabolism Molecular Sequence Data Proto-Oncogene Proteins/genetics,immunology Proto-Oncogene Proteins c-fos Rats Sequence Homology, Nucleic Acid
Chemicals
Antigens Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cohen D R
Department of Molecular Oncology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Curran T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.5.2063-2069.1988
Published
1988-05-00
Pages
2063-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363385
Subset
IM
Databases
GENBANK
M19651
External Links
PubMed source DOI: 10.1128/mcb.8.5.2063-2069.1988 PMC Full Text View journal details
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