Home LiteratureArticle Details

PMID: 3133556 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Detection of fos protein during osteogenesis by monoclonal antibodies.

Molecular and cellular biology ·Vol. 8 ·No. 5 ·1988-05-00 ·Pages 2251-6

De Togni P, Niman H, Raymond V, Sawchenko P, Verma IM

Abstract

We have generated monoclonal antibodies by using a synthetic peptide corresponding to amino acid positions 4 to 17 of the human fos protein. The antibodies detected both v- and c-fos proteins by immunoprecipitation, immunoblotting, and indirect immunofluorescence. The monoclonal antibodies not only identified the fos protein complex with the cellular 39-kilodalton protein, but also recognized the modified forms of the mouse, rat, and human fos proteins. In day-17 rat embryos, nuclear-staining fos protein could be identified in the cartilage by immunohistochemical staining.

MeSH Terms

Animals Antibodies, Monoclonal/immunology Cartilage/analysis,embryology Gene Products, gag Humans Osteogenesis Proto-Oncogene Proteins/analysis,immunology Proto-Oncogene Proteins c-fos Rats Retroviridae Proteins/analysis,immunology

Chemicals

Antibodies, Monoclonal Gene Products, gag Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos Retroviridae Proteins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

De Togni P

Salk Institute, San Diego, California 92138.

Niman H

Raymond V

Sawchenko P

Verma I M

References (26)

26 references, click to expand

Nucleoprotein complexes that regulate gene expression in adipocyte differentiation: direct participation of c-fos.
Cell. 1987 Jun 19;49(6):835-44 PMID: 3555845
Antisense RNA of proto-oncogene c-fos blocks renewed growth of quiescent 3T3 cells.
Mol Cell Biol. 1987 Feb;7(2):639-49 PMID: 3547078
Proto-oncogene c-fos expression in growth regions of fetal bone and mesodermal web tissue.
Nature. 1987 Aug 20-26;328(6132):711-4 PMID: 3614378
The fos oncogene.
Adv Cancer Res. 1987;49:29-52 PMID: 2823543
Proto-oncogene fos: complex but versatile regulation.
Cell. 1987 Nov 20;51(4):513-4 PMID: 3119222
The chicken c-fos gene: cloning and nucleotide sequence analysis.
J Virol. 1987 Dec;61(12):4012-8 PMID: 3316710
Virus induction of osteosarcomas in mice.
Science. 1966 Feb 11;151(3711):698-701 PMID: 5218049
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
Anal Biochem. 1979 Sep 15;98(1):132-5 PMID: 543547
Molecular dissection of Rauscher virus gp70 by using monoclonal antibodies: localization of acquired sequences of related envelope gene recombinants.
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4524-8 PMID: 6933501
Chemically synthesized peptides predicted from the nucleotide sequence of the hepatitis B virus genome elicit antibodies reactive with the native envelope protein of Dane particles.
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3403-7 PMID: 6167985
Analysis of FBJ-MuSV provirus and c-fos (mouse) gene reveals that viral and cellular fos gene products have different carboxy termini.
Cell. 1983 Apr;32(4):1241-55 PMID: 6301687
Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein.
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3183-7 PMID: 6574479
Viral and cellular fos proteins: a comparative analysis.
Cell. 1984 Feb;36(2):259-68 PMID: 6319013
Expression of cellular oncogenes.
Curr Top Microbiol Immunol. 1984;112:73-115 PMID: 6383726
Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
Platelet-derived growth factor induces rapid but transient expression of the c-fos gene and protein.
Nature. 1984 Dec 20-1985 Jan 2;312(5996):711-6 PMID: 6514007
Viral and cellular fos proteins are complexed with a 39,000-dalton cellular protein.
Mol Cell Biol. 1985 Jan;5(1):167-72 PMID: 3885008
General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids.
Proc Natl Acad Sci U S A. 1985 Aug;82(15):5131-5 PMID: 2410914
Anti-peptide antibodies detect oncogene-related proteins in urine.
Proc Natl Acad Sci U S A. 1985 Dec;82(23):7924-8 PMID: 3906653
Cellular and viral fos genes: structure, regulation of expression and biological properties of their encoded products.
Biochim Biophys Acta. 1986;823(3):207-25 PMID: 3011086
Inducible production of c-fos antisense RNA inhibits 3T3 cell proliferation.
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4794-8 PMID: 3523478
Antibody probing of western blots which have been stained with india ink.
Anal Biochem. 1986 Aug 1;156(2):315-9 PMID: 2429580
Deregulated c-fos expression interferes with normal bone development in transgenic mice.
Nature. 1987 Jan 29-Feb 4;325(6103):412-6 PMID: 3027573
Modification of fos proteins: phosphorylation of c-fos, but not v-fos, is stimulated by 12-tetradecanoyl-phorbol-13-acetate and serum.
Mol Cell Biol. 1987 Jun;7(6):2201-11 PMID: 3110603

Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.8.5.2251-2256.1988

Published

1988-05-00

Pages

2251-6

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC363412

Subset

Grants

NCI NIH HHS · CA38160 · United States

NCI NIH HHS · CP41009 · United States

External Links

PubMed source DOI: 10.1128/mcb.8.5.2251-2256.1988 PMC Full Text View journal details

Analysis Services

Detection of fos protein during osteogenesis by monoclonal antibodies.

Contact

WeChat Official Account