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PMID: 3137209 Published · ppublish English Journal Article

Secretion of interleukin-1 beta and Escherichia coli galactokinase by Streptomyces lividans.

Journal of bacteriology ·Vol. 170 ·No. 9 ·1988-09-00 ·Pages 3924-9

Lichenstein H, Brawner ME, Miles LM, Meyers CA, Young PR, Simon PL, Eckhardt T

Abstract

The functionality of the Streptomyces lividans beta-galactosidase signal peptide to direct heterologous protein export was examined. The signal peptide plus eight amino acids of mature protein were sufficient to export not only a naturally exported protein, interleukin-1 beta, but also a naturally occurring cytoplasmic protein, Escherichia coli galactokinase. Interestingly, cells which expressed yet exported galactokinase were phenotypically Gal-. The potential use of the exported galactokinase system for the isolation and characterization of mutations within signal peptides and the export machinery of the host is discussed.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Escherichia coli/enzymology,genetics Galactokinase/metabolism Immunoassay Interleukin-1/metabolism Interleukin-1beta Molecular Sequence Data Peptide Fragments/metabolism Plasmids Protein Sorting Signals/genetics,physiology Streptomyces/enzymology,genetics,metabolism beta-Galactosidase/genetics
Chemicals
Interleukin-1 Interleukin-1beta Peptide Fragments Protein Sorting Signals interleukin-1beta (163-171) Galactokinase beta-Galactosidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lichenstein H
Department of Molecular Genetics, Smith Kline and French Laboratories, King of Prussia, Pennsylvania 19406-0939.
Brawner M E
Miles L M
Meyers C A
Young P R
Simon P L
Eckhardt T
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.9.3924-3929.1988
Published
1988-09-00
Pages
3924-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211391
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.9.3924-3929.1988 PMC Full Text View journal details
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