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PMID: 3137872 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and expression of an alpha-acetolactate decarboxylase gene from Streptococcus lactis subsp. diacetylactis in Escherichia coli.

Applied and environmental microbiology ·Vol. 54 ·No. 7 ·1988-07-00 ·Pages 1889-91

Goelling D, Stahl U

Abstract

The Streptococcus lactis gene coding for alpha-acetolactate decarboxylase (ADC) was cloned in Escherichia coli. Subsequent subcloning in E. coli showed that the ADC gene was located within a 1.3-kilobase DNA fragment. The ADC gene was controlled by its own promoter. Gas chromatography showed that S. lactis and the transformed E. coli strains produced the two optical isomers of acetoin in different ratios.

MeSH Terms
Acetoin/metabolism Carboxy-Lyases/genetics Chromosome Mapping Cloning, Molecular Escherichia coli/enzymology,genetics Genes, Bacterial Lactates/metabolism Lactococcus lactis/enzymology,genetics Stereoisomerism
Chemicals
Lactates alpha-acetolactate Acetoin Carboxy-Lyases acetolactate decarboxylase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goelling D
Technische Universität Berlin, Abteilung Mikrobiologie, Federal Republic of Germany.
Stahl U
References (5)
5 references, click to expand
  1. Acetolactate decarboxylase from Aerobacter aerogenes. Purification and properties.
    Eur J Biochem. 1970 May 1;14(1):133-7 PMID: 5447429
  2. Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid.
    Appl Environ Microbiol. 1978 Mar;35(3):592-600 PMID: 416755
  3. Studies on the formation of acetoin from acetaldehyde by the pyruvate dehydrogenase complex and its regulation.
    Eur J Biochem. 1976 Jul 15;66(3):551-7 PMID: 954756
  4. Isolated tumoral pyruvate dehydrogenase can synthesize acetoin which inhibits pyruvate oxidation as well as other aldehydes.
    Biochem Biophys Res Commun. 1987 May 29;145(1):153-9 PMID: 3593337
  5. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.54.7.1889-1891.1988
Published
1988-07-00
Pages
1889-91
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC202768
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.54.7.1889-1891.1988 PMC Full Text View journal details
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