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PMID: 3138423 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Calibration of the change in thermal stability of DNA duplexes and degree of base pair mismatch.

Journal of molecular evolution ·Vol. 27 ·No. 3 ·1988-00-00 ·Pages 212-6

Caccone A, DeSalle R, Powell JR

Abstract

One method of determining the degree of base pair divergence between two sources of DNA (different strains, species, etc.) is to determine the decrease in thermal stability of hybrid duplex DNA due to mismatching of base pairs. Attempts to calibrate the change in median melting temperature (delta Tm) to base pair mismatch have led to conflicting results. We have studied the delta Tm between DNAs of known sequence over a range of from 0.55% to 7.2% base pair mismatch. The relationship of delta Tm and percent base pair mismatch is remarkably linear over this range with a correlation coefficient greater than 0.98. A delta Tm of 1 degree C corresponds to 1.7% base pair mismatch. This conversion is higher than that usually assumed and, therefore, rates of DNA evolution estimated by DNA-DNA hybridization studies are likely faster than previously thought.

MeSH Terms
Animals Base Composition Cloning, Molecular DNA/genetics DNA, Mitochondrial/genetics Drosophila/genetics Drug Stability Hot Temperature Nucleic Acid Conformation Nucleic Acid Denaturation Nucleic Acid Hybridization RNA, Ribosomal/genetics Thermodynamics
Chemicals
DNA, Mitochondrial RNA, Ribosomal DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Caccone A
Department of Biology, Yale University, New Haven, Connecticut 06511.
DeSalle R
Powell J R
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02100076
Published
1988-00-00
Pages
212-6
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Grants
NIGMS NIH HHS · GM 36648 · United States
External Links
PubMed source DOI: 10.1007/BF02100076 View journal details
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