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PMID: 31407623 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of biomarkers, pathways and potential therapeutic agents for white adipocyte insulin resistance using bioinformatics analysis.

Adipocyte ·Vol. 8 ·No. 1 ·2019-00-00 ·Pages 318-329

Zhang Y, Zheng Y, Fu Y, Wang C

Abstract

For the better understanding of insulin resistance (IR), the molecular biomarkers in IR white adipocytes and its potential mechanism, we downloaded two mRNA expression profiles from Gene Expression Omnibus (GEO). The white adipocyte samples in two databases were collected from the human omental adipose tissue of IR obese (IRO) subjects and insulin-sensitive obese (ISO) subjects, respectively. We identified 86 differentially expressed genes (DEGs) between the IRO and ISO subjects using limma package in R software. Gene Set Enrichment Analysis (GSEA) provided evidence that the most gene sets enriched in kidney mesenchyme development in the ISO subjects, as compared with the IRO subjects. The Gene Ontology (GO) analysis indicated that the most significantly enriched in cellular response to interferon-gamma. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the DEGs were most significantly enriched in cytokine-cytokine receptor interaction. Protein-Protein Interaction (PPI) network was performed with the STRING, and the top 10 hub genes were identified with the Cytohubba. CMap analysis found several small molecular compounds to reverse the altered DEGs, including dropropizine, aceclofenac, melatonin, and so on. Our outputs could empower the novel potential targets to treat omental white adipocyte insulin resistance, diabetes, and diabetes-related diseases.

Keywords
DEGs Insulin resistance bioinformatics hub gene omental adipocyte
MeSH Terms
Adipocytes, White/chemistry Computational Biology/methods Gene Expression Profiling Gene Expression Regulation, Neoplastic Gene Regulatory Networks Genetic Markers Insulin Resistance Obesity/genetics Oligonucleotide Array Sequence Analysis Omentum/chemistry
Chemicals
Genetic Markers
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zhang Yemin
a Department of Pathology and Pathophysiology, Wuhan University School of Basic Medical Sciences , Wuhan , China. | b Hubei Provincial Key Laboratory of Developmentally Originated Disease , Wuhan , China.
Zheng Yuyang
a Department of Pathology and Pathophysiology, Wuhan University School of Basic Medical Sciences , Wuhan , China. | b Hubei Provincial Key Laboratory of Developmentally Originated Disease , Wuhan , China.
Fu Yalin
a Department of Pathology and Pathophysiology, Wuhan University School of Basic Medical Sciences , Wuhan , China. | b Hubei Provincial Key Laboratory of Developmentally Originated Disease , Wuhan , China.
Wang Changhua
a Department of Pathology and Pathophysiology, Wuhan University School of Basic Medical Sciences , Wuhan , China. | b Hubei Provincial Key Laboratory of Developmentally Originated Disease , Wuhan , China.
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Article Info
Journal
Adipocyte
Abbr.
Adipocyte
ISSN
2162-397X
DOI
10.1080/21623945.2019.1649578
Published
2019-00-00
Pages
318-329
Language
English
Region
United States
NLM ID
101567863
PMCID
PMC6768254
Subset
IM
External Links
PubMed source DOI: 10.1080/21623945.2019.1649578 PMC Full Text View journal details
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