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PMID: 3141928 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

cDNA-derived sequence of chicken embryo vinculin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 22 ·1988-11-00 ·Pages 8535-9

Coutu MD, Craig SW

Abstract

We report the complete primary structure of chicken embryo vinculin. The amino acid sequence was derived from the nucleotide sequence of five overlapping cDNA clones isolated from a lambda gt11 phage library. Chicken embryo vinculin contains 1066 amino acids, has a calculated Mr of 116,990, a calculated pI of 5.9, and a hydropathy index of -4.22. A search of the National Biomedical Research Foundation protein sequence data base found no proteins with significant homology to vinculin. A striking feature of the linear sequence is a proline-rich region extending between residues 837 and 879. This region contains 45% proline and 19% aspartic plus glutamic acids; it is also the longest hydrophilic stretch in the molecule. The proline-rich region separates an amino-terminal domain with a calculated pI of 5.4 from a carboxyl-terminal domain with a calculated pI of 9.7. This feature suggests a structural basis for the specific interaction of vinculin with acidic phospholipids and a mechanism for the shuttling of vinculin between cytoplasm and membrane-associated junctional plaque.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Animals Base Sequence Chick Embryo Cloning, Molecular DNA/genetics Molecular Sequence Data Muscle Proteins/genetics Protein Conformation Vinculin
Chemicals
Amino Acids Muscle Proteins Vinculin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Coutu M D
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Craig S W
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.22.8535
Published
1988-11-00
Pages
8535-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282493
Subset
IM
Grants
NIGMS NIH HHS · GM41605 · United States
Databases
GENBANK
J04126
External Links
PubMed source DOI: 10.1073/pnas.85.22.8535 PMC Full Text View journal details
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