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PMID: 3143318 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Specificity of anti-Sm antibodies by ELISA for systemic lupus erythematosus: increased sensitivity of detection using purified peptide antigens.

Annals of the rheumatic diseases ·Vol. 47 ·No. 10 ·1988-10-00 ·Pages 820-5

Field M, Williams DG, Charles P, Maini RN

Abstract

Sm antigen was purified by immunoaffinity chromatography using a murine monoclonal anti-Sm antibody and was confirmed to be free from contaminating polypeptides. This was then used to detect anti-Sm antibodies in patients' sera by enzyme linked immunosorbent assay (ELISA). Antibodies against Sm were detected in only 9/52 (17%) patients with systemic lupus erythematosus (SLE) by immunodiffusion, but 15/52 (29%) were positive for IgG anti-Sm antibodies by ELISA. The presence of anti-Sm antibodies remained disease specific despite the increase in sensitivity of this assay and validates its potential use for clinical application. There was no correlation between the presence of anti-Sm antibodies and any clinical features of SLE. In 23 renal biopsies a membranous component to the glomerulonephritis correlated with anti-Sm antibodies (p less than 0.05). Patients from West Africa, the Carribean Islands, and Asia had a higher prevalence of anti-Sm antibodies than the local Caucasian population.

MeSH Terms
Antibody Specificity Autoantibodies/analysis Autoantigens/analysis,immunology Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Humans Immunodiffusion Kidney/pathology Lupus Erythematosus, Systemic/immunology,pathology Peptides/immunology Ribonucleoproteins, Small Nuclear snRNP Core Proteins
Chemicals
Autoantibodies Autoantigens Peptides Ribonucleoproteins, Small Nuclear snRNP Core Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Field M
Clinical Immunology Division, Kennedy Institute of Rheumatology, Hammersmith, London.
Williams D G
Charles P
Maini R N
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Article Info
Journal
Annals of the rheumatic diseases
Abbr.
Ann Rheum Dis
ISSN
0003-4967
DOI
10.1136/ard.47.10.820
Published
1988-10-00
Pages
820-5
Language
English
Region
England
NLM ID
0372355
PMCID
PMC1003610
Subset
IM
External Links
PubMed source DOI: 10.1136/ard.47.10.820 PMC Full Text View journal details
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