Home LiteratureArticle Details
PMID: 3155721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of the individual regulatory components of the IncFII plasmid replication control system.

Journal of bacteriology ·Vol. 161 ·No. 2 ·1985-02-00 ·Pages 534-43

Womble DD, Dong X, Luckow VA, Wu RP, Rownd RH

Abstract

Replication of the IncFII plasmid NR1 is controlled by regulating the amount of synthesis of the repA1 initiator protein at both the transcriptional and translational levels. We have examined mutations which have altered each of these levels of regulation, resulting in different plasmid copy numbers. The genes which encode each of the individual wild-type or mutant regulatory components from the replication control region of NR1 have been cloned independently into pBR322 vectors, and their effects in trans, either individually or in various combinations, on plasmid incompatibility, stability, copy number, and repA1 gene expression have been defined.

MeSH Terms
Adenosine Triphosphatases/genetics DNA Helicases DNA Replication Gene Expression Regulation Genetic Vectors Mutation Plasmids Transcription, Genetic
Chemicals
Adenosine Triphosphatases DNA Helicases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Womble D D
Dong X
Luckow V A
Wu R P
Rownd R H
References (30)
30 references, click to expand
  1. Post-transcriptional control of expression of the repA gene of plasmid R1 mediated by a small RNA molecule.
    EMBO J. 1983;2(1):93-8 PMID: 11894917
  2. Molecular nature of the drug-resistance factors of the Enterobacteriaceae.
    J Mol Biol. 1966 Jun;17(2):376-93 PMID: 4164241
  3. Round of replication mutant of a drug resistance factor.
    J Bacteriol. 1974 Jun;118(3):855-66 PMID: 4598007
  4. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria.
    Methods Enzymol. 1975;43:737-55 PMID: 1094240
  5. Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.
    J Bacteriol. 1977 Apr;130(1):148-53 PMID: 323223
  6. Generation of miniplasmids from copy number mutants of the R plasmid NR1.
    J Bacteriol. 1977 Dec;132(3):986-95 PMID: 144723
  7. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  8. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  9. Structural and functional analysis of cloned DNA segments containing the replication and incompatibility regions of a miniplasmid derived from a copy number mutant of NR1.
    J Bacteriol. 1979 Jan;137(1):92-104 PMID: 368043
  10. Control of plasmid R1 replication: functions involved in replication, copy number control, incompatibility, and switch-off of replication.
    J Bacteriol. 1980 Jan;141(1):111-20 PMID: 6986353
  11. Cloning of replication, incompatibility, and stability functions of R plasmid NR1.
    J Bacteriol. 1980 Jan;141(1):87-99 PMID: 6243631
  12. Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit beta-globin.
    Cell. 1980 Jun;20(2):543-53 PMID: 6248249
  13. Genes and sites involved in replication and incompatibility of an R100 plasmid derivative based on nucleotide sequence analysis.
    Mol Gen Genet. 1980;179(3):527-37 PMID: 7003300
  14. Role of RNA transcripts in replication incompatibility and copy number control in antibiotic resistance plasmid derivatives.
    Nature. 1981 Apr 30;290(5809):794-7 PMID: 6163994
  15. Regulation of DNA replication: "target" determinant of the replication control elements of plasmid R6-5 lies within a control element gene.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4699-703 PMID: 7029525
  16. RNAs involved in copy-number control and incompatibility of plasmid R1.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6008-12 PMID: 6171808
  17. Replication control functions of plasmid R1 act as inhibitors of expression of a gene required for replication.
    Mol Gen Genet. 1981;184(1):56-61 PMID: 7038389
  18. Expression of a copy number control gene (copB) of plasmid R1 is constitutive and growth rate dependent.
    J Bacteriol. 1982 Sep;151(3):1129-35 PMID: 7050077
  19. Molecular cloning and functional characterization of a copy number control gene (copB) of plasmid R1.
    J Bacteriol. 1982 Sep;151(3):1136-45 PMID: 7050078
  20. The incompatibility product of IncFII R plasmid NR1 controls gene expression in the plasmid replication region.
    J Bacteriol. 1982 Nov;152(2):829-39 PMID: 6290455
  21. The sites of action of the two copy number control functions of plasmid R1.
    Mol Gen Genet. 1982;187(3):486-93 PMID: 6294474
  22. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  23. Replication control mutations of plasmid R6-5 and their effects on interactions of the RNA-I control element with its target.
    J Bacteriol. 1983 Apr;154(1):429-36 PMID: 6187730
  24. The repA2 gene of the plasmid R100.1 encodes a repressor of plasmid replication.
    Plasmid. 1983 Sep;10(2):148-55 PMID: 6356188
  25. Definition of oriR, the minimum DNA segment essential for initiation of R1 plasmid replication in vitro.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6814-8 PMID: 6316326
  26. Construction of a single-copy promoter vector and its use in analysis of regulation of the transposon Tn10 tetracycline resistance determinant.
    J Bacteriol. 1984 Jun;158(3):910-9 PMID: 6327648
  27. Copy mutants of plasmid R1: effects of base pair substitutions in the copA gene on the replication control system.
    Mol Gen Genet. 1984;194(1-2):286-92 PMID: 6203014
  28. IncFII plasmid incompatibility product and its target are both RNA transcripts.
    J Bacteriol. 1984 Oct;160(1):28-35 PMID: 6207169
  29. Regulation of transcription of the repA1 gene in the replication control region of IncFII plasmid NR1 by gene dosage of the repA2 transcription repressor protein.
    J Bacteriol. 1985 Feb;161(2):544-51 PMID: 3155722
  30. Transduction of linked genetic characters of the host by bacteriophage P1.
    Virology. 1955 Jul;1(2):190-206 PMID: 13267987
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.161.2.534-543.1985
Published
1985-02-00
Pages
534-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214915
Subset
IM
Grants
NIGMS NIH HHS · GM30731 · United States
External Links
PubMed source DOI: 10.1128/jb.161.2.534-543.1985 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2