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PMID: 3156204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sera from patients with poststreptococcal glomerulonephritis contain antibodies to glomerular heparan sulfate proteoglycan.

The Journal of experimental medicine ·Vol. 161 ·No. 2 ·1985-02-01 ·Pages 277-89

Fillit H, Damle SP, Gregory JD, Volin C, Poon-King T, Zabriskie J

Abstract

Antibodies, found in human sera from patients with poststreptococcal glomerulonephritis, against proteoglycans (PG) derived from bovine and human glomeruli were investigated. PG were isolated by 4 M guanidine-HCl extraction of whole glomeruli, followed by DEAE-Sepharose CL-6B ion exchange chromatography. The anionic fractions were further purified by chromatography on Sepharose CL-4B. Biochemical analysis of the two resulting peaks revealed the presence of high molecular weight anionic material containing protein, uronic acid, glucosamine, and galactosamine. Enzymatic and chemical susceptibilities indicated the presence of heparan sulfate PG and a galactosamine-containing PG. Immunologic studies revealed the presence of anti-PG antibodies to both PG peaks of the Sepharose CL-4B column in glomerulonephritis sera. Inhibition studies using an ELISA demonstrated that heparan sulfate was a major antigenic determinant. Cross-reactivity with both mammalian and streptococcal hyaluronate was noted. Inhibition studies also indicated the presence of a second antigenic site containing N-acetylgalactosamine, possibly representing chondroitin or dermatan sulfate PG.

MeSH Terms
Acute Disease Animals Antigen-Antibody Reactions Autoantibodies/analysis Cattle Chemical Phenomena Chemistry, Physical Chondroitin Sulfate Proteoglycans/analysis,immunology,isolation & purification Enzyme-Linked Immunosorbent Assay Glomerulonephritis/etiology,immunology Glycosaminoglycans/immunology Heparan Sulfate Proteoglycans Heparitin Sulfate/analysis,immunology,isolation & purification Hexosamines/isolation & purification Humans Kidney Glomerulus/immunology Proteins/isolation & purification Proteoglycans/immunology Rabbits Streptococcal Infections/immunology Uronic Acids/isolation & purification
Chemicals
Autoantibodies Chondroitin Sulfate Proteoglycans Glycosaminoglycans Heparan Sulfate Proteoglycans Hexosamines Proteins Proteoglycans Uronic Acids Heparitin Sulfate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fillit H
Damle S P
Gregory J D
Volin C
Poon-King T
Zabriskie J
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29 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.161.2.277
Published
1985-02-01
Pages
277-89
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187565
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.161.2.277 PMC Full Text View journal details
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