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PMID: 3157991 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Protein kinase C regulates ionic conductance in hippocampal pyramidal neurons: electrophysiological effects of phorbol esters.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 8 ·1985-04-00 ·Pages 2538-42

Baraban JM, Snyder SH, Alger BE

Abstract

The vertebrate central nervous system contains very high concentrations of protein kinase C, a calcium- and phospholipid-stimulated phosphorylating enzyme. Phorbol esters, compounds with inflammatory and tumor-promoting properties, bind to and activate this enzyme. To clarify the role of protein kinase C in neuronal function, we have localized phorbol ester receptors in the rat hippocampus by autoradiography and examined the electrophysiological effects of phorbol esters on hippocampal pyramidal neurons in vitro. Phorbol esters blocked a calcium-dependent potassium conductance. In addition, phorbol esters blocked the late hyperpolarization elicited by synaptic stimulation even though other synaptic potentials were not affected. The potencies of several phorbol esters in exerting these actions paralleled their affinities for protein kinase C, suggesting that protein kinase C regulates membrane ionic conductance.

MeSH Terms
Animals Caenorhabditis elegans Proteins Carrier Proteins Electrophysiology Hippocampus/drug effects,physiology In Vitro Techniques Male Neurons/physiology Phorbol Esters/pharmacology Phorbols/pharmacology Protein Kinase C Protein Kinases/physiology Rats Rats, Inbred Strains Receptors, Drug Receptors, Immunologic/physiology
Chemicals
Caenorhabditis elegans Proteins Carrier Proteins Phorbol Esters Phorbols Receptors, Drug Receptors, Immunologic phorbol ester binding protein phorbol ester receptor Protein Kinases Protein Kinase C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baraban J M
Snyder S H
Alger B E
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.8.2538
Published
1985-04-00
Pages
2538-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397594
Subset
IM
Grants
NIMH NIH HHS · MH-18501 · United States
NINDS NIH HHS · NS-16375 · United States
NINDS NIH HHS · NS-17539 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.8.2538 PMC Full Text View journal details
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