Home LiteratureArticle Details
PMID: 3160660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Opsonophagocytic killing activity of rabbit antibody to Pseudomonas aeruginosa mucoid exopolysaccharide.

Infection and immunity ·Vol. 49 ·No. 2 ·1985-08-00 ·Pages 281-5

Ames P, DesJardins D, Pier GB

Abstract

We used an in vitro opsonophagocytic killing assay to measure the functional activity of antibody directed at the mucoid exopolysaccharide (MEP) antigen expressed by Pseudomonas aeruginosa strains isolated from cystic fibrosis patients. Rabbit antibodies raised to purified MEP were able to mediate phagocytic killing in the presence of human peripheral blood leukocytes and a low level (final concentration, 0.3%) of fresh normal human serum as a complement source. No bacterial killing was observed when peripheral blood leukocytes, antiserum, or complement was omitted. Specificity of the antibody for the MEP antigen was shown by adsorption and inhibition assays. Affinity-purified antibody to MEP also mediate phagocytic killing. These data indicate that antiphagocytic properties attributable to MEP can be overcome by specific antibody.

MeSH Terms
Animals Antibodies, Bacterial/immunology Glycosaminoglycans/immunology Immune Sera Opsonin Proteins/immunology Phagocytosis Pseudomonas aeruginosa/immunology Rabbits/immunology
Chemicals
Antibodies, Bacterial Glycosaminoglycans Immune Sera Opsonin Proteins mucoid exopolysaccharide (P aeruginosa)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ames P
DesJardins D
Pier G B
References (16)
16 references, click to expand
  1. Proteins of the cystic fibrosis respiratory tract. Fragmented immunoglobulin G opsonic antibody causing defective opsonophagocytosis.
    J Clin Invest. 1984 Jul;74(1):236-48 PMID: 6429195
  2. Characterization of the human immune response to a polysaccharide vaccine from Pseudomonas aeruginosa.
    J Infect Dis. 1983 Aug;148(2):206-13 PMID: 6411830
  3. Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using [3H]thymidine labeled bacteria.
    J Immunol Methods. 1977;14(3-4):303-11 PMID: 839081
  4. Immunochemical characterization of the mucoid exopolysaccharide of Pseudomonas aeruginosa.
    J Infect Dis. 1983 Mar;147(3):494-503 PMID: 6220094
  5. Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains.
    Infect Immun. 1983 Oct;42(1):170-7 PMID: 6413410
  6. Human immunity to Pseudomonas aeruginosa. I. In-vitro interaction of bacteria, polymorphonuclear leukocytes, and serum factors.
    J Infect Dis. 1972 Sep;126(3):257-76 PMID: 4626509
  7. Antiphagocytic Effect of Slime from a Mucoid Strain of Pseudomonas aeruginosa.
    Infect Immun. 1971 Jun;3(6):762-7 PMID: 16558051
  8. Cystic fibrosis in adults. 75 cases and a review of 232 cases in the literature.
    Am J Med. 1979 Jan;66(1):121-32 PMID: 420238
  9. Detection of antibodies to Pseudomonas aeruginosa alginate extracellular polysaccharide in animals and cystic fibrosis patients by enzyme-linked immunosorbent assay.
    J Clin Microbiol. 1983 Aug;18(2):276-82 PMID: 6413528
  10. Safety and immunogenicity of high molecular weight polysaccharide vaccine from immunotype 1 Pseudomonas aeruginosa.
    J Clin Invest. 1982 Feb;69(2):303-8 PMID: 6799548
  11. Complement-mediated phagocytosis of Pseudomonas aeruginosa.
    J Lab Clin Med. 1978 Dec;92(6):883-94 PMID: 105067
  12. Antibody to Pseudomonas aeruginosa mucoid exopolysaccharide and to sodium alginate in cystic fibrosis serum.
    Pediatr Res. 1984 May;18(5):431-3 PMID: 6233529
  13. Mediation of the killing of rough, mucoid isolates of Pseudomonas aeruginosa from patients with cystic fibrosis by the alternative pathway of complement.
    J Infect Dis. 1984 Aug;150(2):223-8 PMID: 6432921
  14. Cystic fibrosis pseudomonas opsonins. Inhibitory nature in an in vitro phagocytic assay.
    J Clin Invest. 1981 Oct;68(4):899-914 PMID: 6793632
  15. Polyuronic acids produced by Pseudomonas aeruginosa.
    Biochemistry. 1966 Sep;5(9):2817-22 PMID: 4960208
  16. Nonspecific immunoglobulin synthesis and elevated IgG levels in rabbits immunized with mucoid exopolysaccharide from cystic fibrosis isolates of Pseudomonas aeruginosa.
    J Immunol. 1984 Aug;133(2):734-9 PMID: 6234359
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.49.2.281-285.1985
Published
1985-08-00
Pages
281-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262011
Subset
IM
Grants
PHS HHS · 18465 · United States
External Links
PubMed source DOI: 10.1128/iai.49.2.281-285.1985 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2