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PMID: 3191913 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Physical identification of branched intron side-products of splicing in Trypanosoma brucei.

The EMBO journal ·Vol. 7 ·No. 8 ·1988-08-00 ·Pages 2539-45

Ralph D, Huang J, Van der Ploeg LH

Abstract

Every mRNA in trypanosomes consists of two exons, a common 5' capped mini-exon or spliced leader and a coding-exon. All evidence suggests that the exons are joined by trans-splicing of two individual precursor RNAs, the mini-exon donor RNA or spliced leader precursor RNA (medRNA) and the pre-mRNA. We studied intermediates of the splicing reaction using denaturing two-dimensional PAGE and structurally identified a group of small (approximately 180-300 nt) non-polyadenylated, Y-shaped branched RNAs. The branched Y-shaped RNAs contain the 105 nt medRNA derived intron, joined in a 2'-5' phosphodiester bond to small heterogeneously sized RNAs. These non-polyadenylated branched Y-shaped RNA molecules are analogous to the lariat shaped introns of higher eukaryotes and presumably represent the released intron-like by-products of a trans-splicing reaction which joins the mini-exon and the major coding-exon.

MeSH Terms
Animals Blotting, Northern Electrophoresis, Polyacrylamide Gel Exons HeLa Cells Humans Introns Nucleic Acid Hybridization RNA Precursors/genetics RNA Splicing RNA, Messenger/genetics Transcription, Genetic Trypanosoma brucei brucei/genetics
Chemicals
RNA Precursors RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ralph D
Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Huang J
Van der Ploeg L H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-08-00
Pages
2539-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457125
Subset
IM
Grants
NIAID NIH HHS · AI21784 · United States
External Links
PubMed source PMC Full Text View journal details
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