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PMID: 319338 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Excision-repair in mutants of Escherichia coli deficient in DNA polymerase I and/or its associated 5' leads to 3' exonuclease.

Molecular & general genetics : MGG ·Vol. 150 ·No. 1 ·1977-01-07 ·Pages 1-12

Cooper P

Abstract

The ultraviolet (UV) sensitivity of Escherichia coli mutants deficient in the 5' leads to 3' exonuclease activity of DNA polymerase I is intermediate between that of pol+ strains and mutants which are deficient in the polymerizing activity of pol I (polA1). Like polA1 mutants, the 5'-exonclease deficient mutants exhibit increased UV-induced DNA degradation and increased repair synthesis compared to a pol+ strain, although the increase is not as great as in polA1 or in the conditionally lethal mutant BT4113ts deficient in both polymerase I activities. When dimer excision was measured at UV doses low enough to avoid interference from extensive DNA degradation, all three classes of polymerase I deficient mutants were found to remove dimers efficiently from their DNA. We conclude that enzymes alternative to polymerase I can operate in both the excision and resynthesis steps of excision repair and that substitution for either of the polymerase I functions results in longer patches of repair. A model is proposed detailing the possible events in the alternative pathways.

MeSH Terms
DNA Polymerase I/metabolism DNA Repair DNA, Bacterial/biosynthesis,metabolism DNA-Directed DNA Polymerase/metabolism Escherichia coli/enzymology,metabolism,radiation effects Genes Models, Biological Mutation Pyrimidine Dimers/metabolism Ultraviolet Rays
Chemicals
DNA, Bacterial Pyrimidine Dimers DNA Polymerase I DNA-Directed DNA Polymerase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Cooper P
References (33)
33 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02425319
Published
1977-01-07
Pages
1-12
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02425319
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