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PMID: 319459 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nonsense suppressors of yeast cause osmotic-sensitive growth.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 1 ·1977-01-00 ·Pages 305-9

Singh A

Abstract

Many nonsense suppressors of Saccharomyces cerevisiae cause growth inhibition on hypertonic media. Eight tyrosine-inserting UAA (ochre) suppressors, eight tyrosine-inserting UAG (amber) suppressors, a leucine-inserting UAG suppressor, and a serine-inserting recessive lethal UAG suppressor cause osmotic sensitivity, whereas a serine-inserting UAA suppressor does not cause sensitivity. Although the mechanism is not understood, the growth inhibition of specific suppressors on hypertonic media is correlated with their efficiencies of suppression. This heretofore unknown property of nonsense suppressors is useful for mitotic mapping, selecting tRNA mutants, selecting antisuppressors, and scoring nonsense suppressors.

MeSH Terms
Codon Genotype Leucine/metabolism Saccharomyces cerevisiae/physiology Serine/metabolism Suppression, Genetic Tyrosine/metabolism Water-Electrolyte Balance
Chemicals
Codon Tyrosine Serine Leucine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Singh A
References (18)
18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.1.305
Published
1977-01-00
Pages
305-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393248
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.1.305 PMC Full Text View journal details
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