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PMID: 3198249 Published · ppublish English Journal Article

Fibronectin: source of mannose in a highly purified respiratory mucin.

Inflammation ·Vol. 12 ·No. 5 ·1988-10-00 ·Pages 433-46

Bhattacharyya SN, Kaufman B, Khorrami A, Enriquez JI, Manna B

Abstract

Human bronchial mucin, solubilized in an aqueous solution of sodium azide and protease inhibitors, was purified by molecular sieve chromatography. The mucin was purified by Sepharose 4B and 2B column chromatography. Chemical analyses of this preparation showed a typical mucin profile of amino acids and carbohydrates, except for the presence of an appreciable amount of mannose. Sodium dodecyl sulfate-polyacrylamide gel (5%) electrophoresis of this material showed a high Mr glycoprotein at the top of the gel and two additional bands with mobilities of fibronectin subunits (230 and 210 kD). The fibronectin was separated from the mucin by geletin-Sepharose column chromatography, and the fibronectin eluted from the column was immunologically similar to fibronectin purified from human serum. Ion-exchange chromatography of purified mucin resulted in neutral and acidic fractions. The neutral mucin was the major component. Chemical composition of these two fractions indicated that the amount of threonine, serine, and sialic acid was higher in the acidic fraction, whereas the neutral fraction contained more proline, aspartic acid, leucine, glycine, fucose, and galactose than the acidic fraction.

MeSH Terms
Amino Acids/analysis Bronchitis/metabolism Bronchoalveolar Lavage Fluid/analysis Chromatography, Gel Chronic Disease Electrophoresis, Polyacrylamide Gel Fibronectins/isolation & purification Humans Mannose/isolation & purification Mucins/analysis
Chemicals
Amino Acids Fibronectins Mucins Mannose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bhattacharyya S N
Department of Clinical Investigation, William Beaumont Army Medical Center, El Paso, Texas.
Kaufman B
Khorrami A
Enriquez J I
Manna B
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Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
0360-3997
DOI
10.1007/BF00919437
Published
1988-10-00
Pages
433-46
Language
English
Region
United States
NLM ID
7600105
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00919437 View journal details
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