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PMID: 320198 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Escherichia coli mutants deficient in exonuclease VII.

Journal of bacteriology ·Vol. 129 ·No. 2 ·1977-02-00 ·Pages 934-47

Chase JW, Richardson CC

Abstract

Mutants of Escherichia coli having reduced levels of exonuclease VII activity have been isolated by a mass screening procedure. Nine mutants, five of which are known to be of independent origin, were obtained and designated xse. The defects in these strains lie at two or more loci. One of these loci, xseA, lies in the interval between purG and purC; it is 93 to 97% co-transducible with guaA. The order of the genes in this region is purG-xseA guaA,B-purC. The available data do not allow xseA to be ordered with respect to guaA,B. Exonuclease VII purified from E. coli KLC3 xseA3 is more heat labile than exonuclease VII purified from the parent, E. coli PA610 xse+. Therefore, xseA is the structural gene for exonuclease VII. Mutants with defects in the xseA gene show increased sensitivity to nalidixic acid and have an abnormally high frequency of recombination (hyper-Rec phenotype) as measured by the procedure of Konrad and Lehlman (1974). The hyper-Rec character of xseA strains is approximately one-half that of the polAex1 mutant defective in the 5' leads to 3' hydrolytic activity of deoxyribonucleic acid polymerase I. The double mutant, polAex1 xseA7, is twice as hyper-Rec as the polAex1 mutant alone. The xseA- strains are slightly more sensitive to ultraviolet irradiation than the parent strain. Bacteriophages T7, fd, and lambdared grow normally in xseA- strains.

MeSH Terms
Chromosome Mapping Deoxyribonucleases/biosynthesis Drug Resistance, Microbial Escherichia coli/drug effects,enzymology,radiation effects Exonucleases/biosynthesis Genes Lysogeny Mutation Nalidixic Acid/pharmacology Recombination, Genetic Transduction, Genetic Ultraviolet Rays
Chemicals
Nalidixic Acid Deoxyribonucleases Exonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chase J W
Richardson C C
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58 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.129.2.934-947.1977
Published
1977-02-00
Pages
934-47
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235032
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.129.2.934-947.1977 PMC Full Text View journal details
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