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PMID: 3204123 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Unique distribution of the extracellular matrix component thrombospondin in the developing mouse embryo.

The Journal of cell biology ·Vol. 107 ·No. 6 Pt 2 ·1988-12-00 ·Pages 2737-48

O'Shea KS, Dixit VM

Abstract

Immunocytochemical localization of thrombospondin (TSP), a trimeric glycoprotein constituent of extracellular matrices, produced striking regional and temporal patterns of distribution in the developing mouse embryo. TSP was present in many basement membranes, surrounded epithelial cells, and was associated with peripheral nerve outgrowth. During organogenesis, TSP was also found on the surface of myoblasts and chondroblasts, and TSP was differentially deposited in cortical layers. With differentiation of chondrocytes and myotubes immunoreactivity was decreased, and differential cortical staining was lost. Presence of TSP was associated with morphogenetic processes of proliferation, migration, and intercellular adhesion.

MeSH Terms
Animals Antibody Specificity Autoradiography Basement Membrane/analysis Cell Line Electrophoresis, Polyacrylamide Gel Embryo, Mammalian/analysis Epithelium/analysis Extracellular Matrix/analysis Glycoproteins/analysis Immunoblotting Immunohistochemistry Mice Precipitin Tests Thrombospondins
Chemicals
Glycoproteins Thrombospondins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
O'Shea K S
Department of Anatomy, University of Michigan Medical School, Ann Arbor 48109.
Dixit V M
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.6.2737
Published
1988-12-00
Pages
2737-48
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115645
Subset
IM
Grants
NICHD NIH HHS · HD-23867 · United States
NHLBI NIH HHS · HL-39415 · United States
NINDS NIH HHS · NS-21108 · United States
External Links
PubMed source DOI: 10.1083/jcb.107.6.2737 PMC Full Text View journal details
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