A suitable method for construction and cloning hybrid plasmids containing EcoRI-fragments of E. coli genome.

Molecular & general genetics : MGG ·Vol. 150 ·No. 2 ·1977-01-18 ·Pages 211-9

Kozlov JI, Kalinina NA, Gening LV, Rebentish BA, Strongin AY, Bogush VG, Debabov VG

Abstract

A convenient procedure for the isolation of specific EcoRI-fragments of E. coli genome and their amplification on Km-resistance plasmid victor CK delta11 is described. The hybrid molecules were constructued in vitro using EcoRI-digestion, followed by ligation. Then appropriated E. coli strain was transformed with ligated DNA mixture and hybrid plasmids CK delta11-arg+, CK delta11-his+, CK delta11-thr+ and CK delta11-leu+ containing loci of E. coli genome were selected by molecular cloning. The hybrid plasmids obtained consisted of one EcoRI-fragment of initial plasmid CK delta11 and one respective specific portion of E. coli genome.

MeSH Terms

Arginine/metabolism DNA Replication Escherichia coli Extrachromosomal Inheritance Genes Genetic Techniques Histidine/metabolism Hybridization, Genetic Leucine/metabolism Plasmids Threonine/metabolism

Chemicals

Threonine Histidine Arginine Leucine

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Kozlov J I

Kalinina N A

Gening L V

Rebentish B A

Strongin A Y

Bogush V G

Debabov V G

References (14)

14 references, click to expand

Construction of biologically functional bacterial plasmids in vitro.
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
Biochemical construction and selection of hybrid plasmids containing specific segments of the Escherichia coli genome.
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4361-5 PMID: 1105581
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
The nature of the transformation process in Escherichia coli K12.
Mol Gen Genet. 1973 Jul 31;124(1):1-10 PMID: 4582298
Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids.
Cell. 1975 Oct;6(2):231-44 PMID: 1102112
Characterization of six cloned DNAs from Drosophila melanogaster, including one that contains the genes for rRNA.
Cell. 1975 Jun;5(2):149-57 PMID: 806347
Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.
Biochemistry. 1973 Dec 4;12(25):5045-50 PMID: 4366077
Electrophoretic separation of Bacillus subtilis genes.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2207-11 PMID: 806078
Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
Ligation of EcoRI endonuclease-generated DNA fragments into linear and circular structures.
J Mol Biol. 1975 Jul 25;96(1):171-84 PMID: 169355
A reliable technique for large-scale DNA separation.
Anal Biochem. 1977 May 1;79(1-2):1-10 PMID: 326089
Formation of complex Col E1 DNA by replication.
Biochim Biophys Acta. 1971 Feb 25;232(1):32-42 PMID: 4930204
Calcium-dependent bacteriophage DNA infection.
J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli.
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2904-9 PMID: 4342968

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00695401

Published

1977-01-18

Pages

211-9

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00695401 免费全文

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A suitable method for construction and cloning hybrid plasmids containing EcoRI-fragments of E. coli genome.

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