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PMID: 3207762 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Purification methods for the sequence-specific DNA-binding protein nuclear factor I (NFI)--generation of protein sequence information.

Biochimica et biophysica acta ·Vol. 951 ·No. 2-3 ·1988-12-20 ·Pages 411-8

Gander I, Foeckler R, Rogge L, Meisterernst M, Schneider R, Mertz R, Lottspeich F, Winnacker EL

Abstract

The paper describes a potent purification method, preparative gel retention, for the purification of sequence-specific DNA-binding proteins. This procedure exploits the sequence-specific DNA-binding affinity of such proteins for their enrichment, comparable to recognition site DNA affinity chromatography. The method was employed to obtain a pure preparation of nuclear factor I (NFI) from porcine liver from which sequences of partial peptides could be obtained. Oligonucleotide probes derived from these amino-acid sequences were used to identify genomic and cDNA clones of NFI.

MeSH Terms

Amino Acid Sequence Animals Base Sequence CCAAT-Enhancer-Binding Proteins Cell Nucleus/analysis Chromatography, Affinity Chromatography, DEAE-Cellulose Chromatography, Gel Chromatography, High Pressure Liquid DNA/genetics DNA-Binding Proteins/isolation & purification Electrophoresis, Polyacrylamide Gel Liver/analysis Macromolecular Substances Molecular Sequence Data Molecular Weight NFI Transcription Factors Nuclear Proteins Oligonucleotide Probes Swine Transcription Factors Y-Box-Binding Protein 1

Chemicals

CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins Macromolecular Substances NFI Transcription Factors Nuclear Proteins Oligonucleotide Probes Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human DNA

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Gander I

Institut für Biochemie der Universität München, F.R.G.

Foeckler R

Rogge L

Meisterernst M

Schneider R

Mertz R

Lottspeich F

Winnacker E L

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0167-4781(88)90114-5

Published

1988-12-20

Pages

411-8

Language

English

Region

Netherlands

NLM ID

0217513

Subset

External Links

PubMed source DOI: 10.1016/0167-4781(88)90114-5

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Purification methods for the sequence-specific DNA-binding protein nuclear factor I (NFI)--generation of protein sequence information.

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