Home LiteratureArticle Details
PMID: 3211138 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA sequences involved in transcriptional regulation of the mouse beta-globin promoter in murine erythroleukemia cells.

Molecular and cellular biology ·Vol. 8 ·No. 8 ·1988-08-00 ·Pages 3122-8

Cowie A, Myers RM

Abstract

We have developed a transient assay in murine erythroleukemia (MEL) cells to analyze the cis-acting sequence requirements for transcriptional regulation of the mouse beta-major-globin promoter. From deletion analysis, a fragment of the promoter region, from -106 to +26 relative to the RNA cap site, was found to be sufficient for regulated transcription in MEL cells following induction of differentiation by dimethyl sulfoxide. Single-base mutational analysis of this 132-base-pair promoter fragment identified three sequence elements required for transcription in MEL cells. These are the ATATAA sequence at -31 to -26, the CCAATC sequence between -77 and -72, and the GCCACACCC sequence between -95 and -87. In addition, we found a requirement for sequences adjacent to the CCAAT and ATATAA consensus motifs. Point mutations within the promoter did not abolish transcriptional regulation following induction of differentiation by dimethyl sulfoxide. However, mutations that resulted in reduced transcription levels in uninduced MEL cells gave similarly decreased levels in induced MEL cells.

MeSH Terms
Animals Base Sequence Cell Line Chromosome Deletion Gene Expression Regulation Genes Globins/genetics Leukemia, Erythroblastic, Acute/metabolism Mice Molecular Sequence Data Mutation Promoter Regions, Genetic Transcription, Genetic
Chemicals
Globins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cowie A
Department of Physiology, University of California, San Francisco 94143.
Myers R M
References (37)
37 references, click to expand
  1. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.
    Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82 PMID: 5277089
  2. The human beta-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers.
    EMBO J. 1988 Feb;7(2):377-84 PMID: 2835225
  3. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.
    Cell. 1978 Jul;14(3):725-31 PMID: 210957
  4. Human alpha-globin gene expression following chromosomal dependent gene transfer into mouse erythroleukemia cells.
    Cell. 1978 Sep;15(1):55-63 PMID: 279411
  5. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  6. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  7. The structure and evolution of the human beta-globin gene family.
    Cell. 1980 Oct;21(3):653-68 PMID: 6985477
  8. Stability of globin mRNA in terminally differentiating murine erythroleukemia cells.
    Cell. 1981 Feb;23(2):509-14 PMID: 6937265
  9. The primary transcription unit of the mouse beta-major globin gene.
    Cell. 1981 Feb;23(2):585-93 PMID: 6937266
  10. Structure of mouse metallothionein-I gene and its mRNA.
    Nature. 1981 Jul 16;292(5820):267-9 PMID: 7254320
  11. DNA sequences necessary for transcription of the rabbit beta-globin gene in vivo.
    Nature. 1982 Jan 14;295(5845):120-6 PMID: 6276753
  12. Murine erythroleukemia cell differentiation: DNase I hypersensitivity and DNA methylation near the globin genes.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1180-4 PMID: 6280172
  13. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  14. Sequence requirements for the transcription of the rabbit beta-globin gene in vivo: the -80 region.
    Nucleic Acids Res. 1982 Aug 25;10(16):4951-71 PMID: 6290997
  15. 5' termini of polyoma virus early region transcripts synthesized in vivo by wild-type virus and viable deletion mutants.
    J Mol Biol. 1982 Aug 5;159(2):189-224 PMID: 6292432
  16. The regulated expression of beta-globin genes introduced into mouse erythroleukemia cells.
    Cell. 1983 Feb;32(2):483-93 PMID: 6572107
  17. Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells.
    Cell. 1983 Mar;32(3):695-706 PMID: 6299573
  18. Regulated expression of the human beta-globin gene family in murine erythroleukaemia cells.
    Nature. 1983 Sep 22-28;305(5932):333-6 PMID: 6578416
  19. Regulated expression of cloned globin genes introduced into mouse erythroleukemia cells.
    Prog Clin Biol Res. 1983;134:3-12 PMID: 6582520
  20. A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.
    Cell. 1984 May;37(1):273-83 PMID: 6722872
  21. Differences in human alpha- and beta-globin gene expression in mouse erythroleukemia cells: the role of intragenic sequences.
    Cell. 1984 Aug;38(1):251-63 PMID: 6205764
  22. DNA sequences required for regulated expression of beta-globin genes in murine erythroleukemia cells.
    Cell. 1984 Aug;38(1):265-73 PMID: 6088069
  23. G to A substitution in the distal CCAAT box of the A gamma-globin gene in Greek hereditary persistence of fetal haemoglobin.
    Nature. 1985 Jan 24-30;313(6000):323-5 PMID: 2578619
  24. A point mutation in the A gamma-globin gene promoter in Greek hereditary persistence of fetal haemoglobin.
    Nature. 1985 Jan 24-30;313(6000):325-6 PMID: 2578620
  25. A general method for saturation mutagenesis of cloned DNA fragments.
    Science. 1985 Jul 19;229(4710):242-7 PMID: 2990046
  26. Linker scanning mutagenesis of the 5'-flanking region of the mouse beta-major-globin gene: sequence requirements for transcription in erythroid and nonerythroid cells.
    Mol Cell Biol. 1985 Jun;5(6):1498-511 PMID: 3861935
  27. Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences.
    Cell. 1985 Oct;42(3):889-902 PMID: 2414012
  28. Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
    Cell. 1985 Nov;43(1):165-75 PMID: 4075392
  29. Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene.
    Cell. 1986 Feb 28;44(4):565-76 PMID: 3004739
  30. Fine structure genetic analysis of a beta-globin promoter.
    Science. 1986 May 2;232(4750):613-8 PMID: 3457470
  31. A transcriptional enhancer with specificity for erythroid cells is located in the long terminal repeat of the Friend murine leukemia virus.
    EMBO J. 1986 Jul;5(7):1615-23 PMID: 3462001
  32. Transcriptional selectivity of viral genes in mammalian cells.
    Cell. 1986 Sep 12;46(6):795-805 PMID: 3530495
  33. A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication.
    Cell. 1987 Jan 16;48(1):79-89 PMID: 3024847
  34. Human globin gene promoter sequences are sufficient for specific expression of a hybrid gene transfected into tissue culture cells.
    Mol Cell Biol. 1987 Jan;7(1):398-402 PMID: 3561396
  35. A multiplicity of CCAAT box-binding proteins.
    Cell. 1987 Sep 11;50(6):863-72 PMID: 3476205
  36. In vitro RNA synthesis with SP6 RNA polymerase.
    Methods Enzymol. 1987;155:397-415 PMID: 2828872
  37. Erythroleukemic differentiation.
    Annu Rev Biochem. 1978;47:419-48 PMID: 354501
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.8.3122-3128.1988
Published
1988-08-00
Pages
3122-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363539
Subset
IM
Grants
NIGMS NIH HHS · 5R01GM36940-02 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.8.3122-3128.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2