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PMID: 32127635 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish.

Communications biology ·Vol. 3 ·No. 1 ·2020-00-03 ·Pages 94

Ren F, Lin Q, Gong G, Du X, Dan H, Qin W, Miao R, Xiong Y, Xiao R, Li X, Gui JF, Mei J

Abstract

Early embryogenesis relies on maternally inherited mRNAs. Although the mechanism of maternal mRNA degradation during maternal-to-zygotic transition (MZT) has been extensively studied in vertebrates, how the embryos maintain maternal mRNA stability remains unclear. Here, we identify Igf2bp3 as an important regulator of maternal mRNA stability in zebrafish. Depletion of maternal igf2bp3 destabilizes maternal mRNAs prior to MZT and leads to severe developmental defects, including abnormal cytoskeleton organization and cell division. However, the process of oogenesis and the expression levels of maternal mRNAs in unfertilized eggs are normal in maternal igf2bp3 mutants. Gene ontology analysis revealed that these functions are largely mediated by Igf2bp3-bound mRNAs. Indeed, Igf2bp3 depletion destabilizes while its overexpression enhances its targeting maternal mRNAs. Interestingly, igf2bp3 overexpression in wild-type embryos also causes a developmental delay. Altogether, these findings highlight an important function of Igf2bp3 in controlling early zebrafish embryogenesis by binding and regulating the stability of maternal mRNAs.

MeSH Terms
Animals Animals, Genetically Modified Embryo, Nonmammalian Embryonic Development/genetics Gene Expression Regulation, Developmental RNA Stability/genetics RNA, Messenger, Stored/genetics,metabolism RNA-Binding Proteins/genetics,physiology Zebrafish/embryology,genetics,metabolism Zebrafish Proteins/genetics,physiology Zygote/metabolism
Chemicals
RNA, Messenger, Stored RNA-Binding Proteins Zebrafish Proteins igf2bp3 protein, zebrafish
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Ren Fan ORCID
College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, China.
Lin Qiaohong ORCID
College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, China.
Gong Gaorui ORCID
College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, China.
Du Xian ORCID
Frontier Science Center for Immunology and Metabolism, Medical Research Institute, and Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan University, 430071, Wuhan, China.
Dan Hong ORCID
College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, China.
Qin Wenying ORCID
Frontier Science Center for Immunology and Metabolism, Medical Research Institute, and Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan University, 430071, Wuhan, China.
Miao Ran ORCID
College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, China.
Xiong Yang ORCID
College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, China.
Xiao Rui
Frontier Science Center for Immunology and Metabolism, Medical Research Institute, and Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan University, 430071, Wuhan, China.
Li Xiaohui ORCID
College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, China.
Gui Jian-Fang ORCID
College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, China. | State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, 430072, Wuhan, China.
Mei Jie ORCID
College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, China. [email protected].
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Article Info
Journal
Communications biology
Abbr.
Commun Biol
ISSN
2399-3642
DOI
10.1038/s42003-020-0827-2
Email
[email protected]
Published
2020-00-03
Epub
2020-00-03
Pages
94
Language
English
Region
England
NLM ID
101719179
PMCID
PMC7054421
Subset
IM
External Links
PubMed source DOI: 10.1038/s42003-020-0827-2 PMC Full Text View journal details
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