Rapid speciation of Haemophilus with the porphyrin production test versus the satellite test for X.

Journal of clinical microbiology ·Vol. 5 ·No. 2 ·1977-02-00 ·Pages 142-4

Lund ME, Blazevic DJ

Abstract

The detection of porphyrins produced from omega-aminolevulinic acid was evaluated for use in speciation of the Haemophilus. Two hundred and forty-eight clinical isolates of nonhemolytic Haemophilus were tested concurrently for porphyrin production and for X and V requirements. The porphyrin production test was more rapid (4 h) and more accurate than the satellite test.

MeSH Terms

Aminolevulinic Acid/metabolism Bacteriological Techniques Evaluation Studies as Topic Haemophilus/isolation & purification,metabolism Haemophilus influenzae/isolation & purification,metabolism Hemin/metabolism Porphyrins/biosynthesis Species Specificity

Chemicals

Porphyrins Hemin Aminolevulinic Acid

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lund M E

Blazevic D J

References (10)

10 references, click to expand

CATALASE ACTIVITY OF HAEMOPHILUS SPECIES GROWN WITH GRADED AMOUNTS OF HEMIN.
J Bacteriol. 1961 Mar;81(3):380-4 PMID: 16561892
HEMIN BIOSYNTHESIS IN HAEMOPHILUS.
J Bacteriol. 1963 Apr;85:842-50 PMID: 14044953
Some growth requirements of Haemophilus influenzas and Haemophilus pertussis.
J Pathol Bacteriol. 1959 Jan;77(1):95-100 PMID: 13621337
A taxonomic study of the genus Haemophilus, with the proposal of a new species.
J Gen Microbiol. 1976 Mar;93(1):9-62 PMID: 772168
The effect of the medium and source of growth factors on the satellitism test for Haemophilus species.
J Med Microbiol. 1972 Nov;5(4):509-14 PMID: 4345767
The inhibition of Haemophilus influenzae by certain agar and peptone preparations.
J Med Microbiol. 1974 May;7(2):305-10 PMID: 4545831
A rapid method for the differentiation of Haemophilus strains. The porphyrin test;.
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Dec;82(6):835-42 PMID: 4533223
New satellitism test for isolation and identification of Haemophilus influenzae and Haemophilus parainfluenzae in sputum.
J Clin Microbiol. 1975 Jan;1(1):89-95 PMID: 170304
Optimal bacitracin concentration for selective isolation medium for haemophilus.
Am J Med Technol. 1971 Jul;37(7):304-5 PMID: 4937723
Differentiation of haemophilus species using satellite methods.
Am J Med Technol. 1969 Nov;35(11):695-701 PMID: 4899742

Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.5.2.142-144.1977

Published

1977-02-00

Pages

142-4

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC274554

Subset

External Links

PubMed source DOI: 10.1128/jcm.5.2.142-144.1977 PMC Full Text View journal details

Analysis Services

Rapid speciation of Haemophilus with the porphyrin production test versus the satellite test for X.

Contact

WeChat Official Account