A shorter immunoperoxidase technique for the demonstration of carcinoembryonic antigen and other cell products.

Journal of clinical pathology ·Vol. 30 ·No. 2 ·1977-02-00 ·Pages 138-40

Heyderman E, Neville AM

Abstract

A reliable immunoperoxidase schedule for the cellular demonstration of tumour and normal cell products which can be completed in well under two hours is described. The preparations are as clear and readable as those stained by routine histological techniques and should form a valuable adjunct in research and diagnostic studies.

MeSH Terms

Calcitonin/analysis Carcinoembryonic Antigen/analysis Hepatitis B Antigens/analysis Hormones/analysis Humans Immunoenzyme Techniques Immunoglobulins/analysis Muramidase/analysis Neoplasms/analysis,immunology alpha 1-Antitrypsin/analysis

Chemicals

Carcinoembryonic Antigen Hepatitis B Antigens Hormones Immunoglobulins alpha 1-Antitrypsin Calcitonin Muramidase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Heyderman E

Neville A M

References (13)

13 references, click to expand

DEMONSTRATION OF TUMOR-SPECIFIC ANTIGENS IN HUMAN COLONIC CARCINOMATA BY IMMUNOLOGICAL TOLERANCE AND ABSORPTION TECHNIQUES.
J Exp Med. 1965 Mar 1;121:439-62 PMID: 14270243
Inhibition of erythrocyte pseudoperoxidase activity by treatment with hydrogen peroxide following methanol.
J Histochem Cytochem. 1972 Oct;20(10):829-31 PMID: 4118124
Letter: Destruction of endogenous peroxidase activity in order to locate cellular antigens by peroxidase-labeled antibodies.
J Histochem Cytochem. 1974 Jan;22(1):51-4 PMID: 4133071
The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.
J Histochem Cytochem. 1970 May;18(5):315-33 PMID: 4192899
Carcinoembryonic antigen (CEA) in gastrointestinal and extragastrointestinal tumors and its relationship to tumor-cell differentiation.
Int J Cancer. 1972 Sep 15;10(2):262-72 PMID: 4350187
Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material.
Histochemistry. 1975 Jun 5;43(3):291-4 PMID: 50308
Immunological study of carcinoembryonic antigen (CEA) and a related glycoprotein.
Br J Cancer. 1973 Aug;28(2):147-60 PMID: 4199743
Peroxidase-labeled antibody. A new method of conjugation.
J Histochem Cytochem. 1974 Dec;22(12):1084-91 PMID: 4443551
Carcinoembryonic antigenic activity of tissue extracts: a quantitative study of malignant and benign neoplasms, cirrhotic liver, normal adult and fetal organs.
Int J Cancer. 1973 May;11(3):681-7 PMID: 4791225
Studies on carcinoembryonic antigen (CEA) and a related glycoprotein, CCEA-2. Preparation and chemical characterisation.
Immunochemistry. 1973 Dec;10(12):841-3 PMID: 4767435
Histochemical use of borohydrides as aldehyde blocking reagents.
Stain Technol. 1972 Jan;47(1):13-6 PMID: 5008771
Detection of carcinoembryonic antigen in tissue sections by immunoperoxidase.
J Immunol Methods. 1975 Sep;8(3):267-75 PMID: 1237519
An optical and ultrastructural study of the localization of carcinoembryonic antigen (CEA) in normal and cancerous human rectocolonic mucosa.
Lab Invest. 1976 Jan;34(1):97-107 PMID: 1246127

Article Info

Journal

Journal of clinical pathology

Abbr.

J Clin Pathol

ISSN

0021-9746

DOI

10.1136/jcp.30.2.138

Published

1977-02-00

Pages

138-40

Language

English

Region

England

NLM ID

0376601

PMCID

PMC476336

Subset

External Links

PubMed source DOI: 10.1136/jcp.30.2.138 PMC Full Text View journal details

Analysis Services

A shorter immunoperoxidase technique for the demonstration of carcinoembryonic antigen and other cell products.

Contact

WeChat Official Account