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PMID: 321699 Published · ppublish English Journal Article

Pronase pretreatment of tissue sections enhances sensitivity of the unlabelled antibody-enzyme (PAP) technique.

Journal of immunological methods ·Vol. 15 ·No. 2 ·1977-00-00 ·Pages 163-7

Denk H, Radaszkiewicz T, Weirich E

Abstract

Treatment of dewaxed sections of formalin-fixed paraffin embedded material with 0.1% pronase for 15 min at 37 degrees C prior to the performance of the unlabelled antibody-enzyme (PAP) procedure greatly enhanced its sensitivity. This was shown by detection of immunoglobulin within plasma cells in chronic gastritis specimens.

MeSH Terms
Animals Formaldehyde/metabolism Gastric Mucosa/immunology Humans Immunoenzyme Techniques Pronase/metabolism Rabbits
Chemicals
Formaldehyde Pronase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Denk H
Radaszkiewicz T
Weirich E
Article Info
Journal
Journal of immunological methods
Abbr.
J Immunol Methods
ISSN
0022-1759
DOI
10.1016/0022-1759(77)90027-8
Published
1977-00-00
Pages
163-7
Language
English
Region
Netherlands
NLM ID
1305440
Subset
IM
External Links
PubMed source DOI: 10.1016/0022-1759(77)90027-8 View journal details
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