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PMID: 3217229 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparison of Ca2+-dependent K+ channels in the membrane of smooth muscle cells isolated from adult and foetal human aorta.

Pflugers Archiv : European journal of physiology ·Vol. 413 ·No. 1 ·1988-11-00 ·Pages 8-13

Bregestovski PD, Printseva OYu, Serebryakov V, Stinnakre J, Turmin A, Zamoyski V

Abstract

Ca2+-activated K+ ionic currents in the membrane of cultured smooth muscle cells isolated from foetal and adult human aorta were studied using whole cell and single-channel patch-clamp techniques. Whole cell currents in adult smooth muscle cells were 3-8 times larger than in foetal cells of similar sizes. The elementary conductance and ionic selectivity of single Ca2+-activated K+ were identical for both types of cells. Channel openings occurred in burst, the duration of which was 3-5-fold longer in adult than in foetal cells. The voltage dependency of the channel activating mechanism and the dependency of the mean open time on the Ca2+ concentration on the inner side of the membrane were similar for both types of cells. These results suggest that the main reason for the increase in potassium conductance during development is an alteration in the open time of the Ca2+-activated K+ channels.

MeSH Terms
Adult Aorta/metabolism,physiology Calcium/metabolism Cells, Cultured Fetus/metabolism Humans Membrane Potentials Middle Aged Muscle, Smooth, Vascular/metabolism,physiology Potassium Channels/metabolism
Chemicals
Potassium Channels Calcium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bregestovski P D
Institute of Experimental Cardiology, Research Centre of the USSR Academy of Medical Sciences, Moscow.
Printseva OYu
Serebryakov V
Stinnakre J
Turmin A
Zamoyski V
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00581222
Published
1988-11-00
Pages
8-13
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00581222 View journal details
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