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PMID: 321805 Published · ppublish English Journal Article

In vivo effects of recBC DNase, exonuclease I, and DNA polymerases of Escherichia coli on the infectivity of native and single-stranded DNA of bacteriophage T7.

Journal of virology ·Vol. 21 ·No. 3 ·1977-03-00 ·Pages 906-12

Seroka K, Wackernagel W

Abstract

The effect of several enzymes of the DNA metabolism of Escherichia coli on the biological activity of native and single-stranded T7 DNA was studied by transfection of lysozyme-EDTA spheroplasts prepared from various E. coli mutants. It is shown that the presence of the recBC DNase in the recipient cells decreases the infectivity of native and denatured DNA by about 100- and 10-fold, respectively. Lack of exonuclease I did not stimulate transfection by single-stranded DNA. Separated light (l) and heavy (r) strands of T7 DNA are fully infective, with a linear dependence on DNA concentrations, whereas heat-denatured DNA shows a two-hit kinetics. Single-stranded DNA was observed to depend on a functional DNA polymerase III for infectivity in polAB cells, whereas transfection with native T7 DNA was independent of the host DNA polymerases. The results are discussed with respect to the mode of T7 DNA replication.

MeSH Terms
Coliphages/metabolism DNA, Single-Stranded/metabolism DNA, Viral/metabolism DNA-Directed DNA Polymerase/metabolism Deoxyribonucleases/metabolism Escherichia coli/enzymology Exonucleases/metabolism Hot Temperature Mutation Nucleic Acid Denaturation Spheroplasts/enzymology Transformation, Genetic
Chemicals
DNA, Single-Stranded DNA, Viral DNA-Directed DNA Polymerase Deoxyribonucleases Exonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Seroka K
Wackernagel W
References (39)
39 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.21.3.906-912.1977
Published
1977-03-00
Pages
906-12
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515628
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.21.3.906-912.1977 PMC Full Text View journal details
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