A new technique for selection of sensitive and auxotrophic mutants of E. coli: isolation of a strain sensitive to an excess of one-carbon metabolites.

Molecular & general genetics : MGG ·Vol. 150 ·No. 3 ·1977-02-15 ·Pages 293-9

Danchin A

Abstract

An E. coli strain deleted in the region malasd is used for the selection of conditional or auxotrophic mutants. Thermosensitive and auxotrophic strains have thus been isolated on plates. After selection in liquid medium, a strain has been isolated which is sensitive to excess one-carbon metabolites. It carries two mutations, smg A1 (near metA and arg H), probably identical to relC, and smgB (between asn and ilv), probably part of the E. coli membrane ATPase.

MeSH Terms

Escherichia coli/isolation & purification,metabolism Glycine/metabolism Methionine/metabolism Mutation Protein Biosynthesis Serine/metabolism Species Specificity Temperature Transcription, Genetic

Chemicals

Serine Methionine Glycine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Danchin A

References (7)

7 references, click to expand

Does formylation of initiator tRNA act as a regulatory signal in E. coli?
FEBS Lett. 1973 Aug 15;34(2):327-32 PMID: 4355917
Mutants of Escherichia coli K12 unable to grow on non-fermentable carbon substrates.
Biochim Biophys Acta. 1975 Feb 17;376(2):195-209 PMID: 234746
A rapid test for the rel A mutation in E. coli.
Biochem Biophys Res Commun. 1976 Apr 5;69(3):751-8 PMID: 773380
Potassium transport loci in Escherichia coli K-12.
J Bacteriol. 1971 Nov;108(2):639-44 PMID: 4942756
Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12.
J Bacteriol. 1973 Feb;113(2):798-812 PMID: 4570607
Recalibrated linkage map of Escherichia coli K-12.
Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00268128

Published

1977-02-15

Pages

293-9

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00268128

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A new technique for selection of sensitive and auxotrophic mutants of E. coli: isolation of a strain sensitive to an excess of one-carbon metabolites.

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