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PMID: 322137 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Localization of bovine brain filament antibody on intermediate (100 A) filaments in guinea pig vascular endothelial cells and chick cardiac muscle cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 2 ·1977-02-00 ·Pages 662-5

Blose SH, Shelanski ML, Chacko S

Abstract

Guinea pig vascular endothelial cells contain naturally occurring rings of intermediate filaments that completely encircle the nucleus. Indirect immunofluorescence staining showed that these perinuclear rings bound antibody prepared against protein from bovine brain 9-nm filaments. In endothelial cells grown in the presence of 1 muM demecolcine (Colcemid) the perinuclear ring "coils" into a juxtanuclear "cap". Throughout this process we could demonstrate staining of the intermediate filaments. Chick cardiac muscle cells in culture stained diffusely with the antibody. After treatment for 24 hr with 1 muM demecolcine the cardiac cells accumulated large bands of intermediate filaments. These bands stained intensely with the antibody. Our findings suggest that intermediate filaments in guinea pig endothelial cells and those induced in chick cardiac muscle cells are antigenically similar to bovine brain filaments. The staining of these filaments is not affected by treatment with demecolcine.

MeSH Terms
Animals Antibodies Aorta, Thoracic/immunology,ultrastructure Chickens Demecolcine/pharmacology Endothelium/drug effects,immunology,ultrastructure Fluorescent Antibody Technique Guinea Pigs Microscopy, Electron Microscopy, Phase-Contrast Muscle Proteins/immunology Myocardium/immunology,ultrastructure Nerve Tissue Proteins/immunology Portal Vein/immunology,ultrastructure
Chemicals
Antibodies Muscle Proteins Nerve Tissue Proteins Demecolcine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blose S H
Shelanski M L
Chacko S
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.2.662
Published
1977-02-00
Pages
662-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392352
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.2.662 PMC Full Text View journal details
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