A new method for the unidirectional deletion of DNA with Bal 31 nuclease using 2'-O-MeRNA-DNA chimeric adaptors.

Nucleic acids symposium series ·No. 19 ·1988-00-00 ·Pages 117-20

Mukai S, Shibahara S, Morisawa H

Abstract

We have developed a new procedure for unidirectional DNA deletion with Bal 31 nuclease. This method is based on the use of chimeric adaptors containing double strands of 2'-O-methyl RNA to block against an attack of Bal 31 nuclease. This contributes a wide application to learn the relation of structure-function of genes.

MeSH Terms

Chimera Chromosome Deletion DNA/genetics Endodeoxyribonucleases Genes Genetic Engineering/methods Methylation RNA, Double-Stranded

Chemicals

RNA, Double-Stranded DNA Endodeoxyribonucleases exonuclease Bal 31

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Mukai S

Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.

Shibahara S

Morisawa H

Article Info

Journal

Nucleic acids symposium series

Abbr.

Nucleic Acids Symp Ser

ISSN

0261-3166

Published

1988-00-00

Pages

117-20

Language

English

Region

England

NLM ID

8007206

Subset

External Links

PubMed source

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A new method for the unidirectional deletion of DNA with Bal 31 nuclease using 2'-O-MeRNA-DNA chimeric adaptors.

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