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PMID: 3239778 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod.

Analytical biochemistry ·Vol. 175 ·No. 2 ·1988-12-00 ·Pages 525-30

Monsigny M, Petit C, Roche AC

Abstract

A microscale colorimetric assay for neutral sugars, in which neutral sugars react with resorcinol in the presence of 75% sulfuric acid solution is described. This assay, based on the use of microtiter plates with 96 U-shaped wells is simple and easy to handle; it allows accurate determinations with small samples (20 microliters) containing 1 to 100 nmol of neutral sugars and is quite convenient for detection of glycoconjugates in chromatographic column effluents.

MeSH Terms

Carbohydrates/analysis Colorimetry/methods Glycoconjugates/analysis Hexoses/analysis Indicators and Reagents Mannose/analysis Microchemistry Resorcinols Sulfuric Acids

Chemicals

Carbohydrates Glycoconjugates Hexoses Indicators and Reagents Resorcinols Sulfuric Acids Mannose resorcinol

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Monsigny M

Laboratoire de Biochimie Cellulaire et Moléculaire des Glycoconjugués, Centre National de la Recherche Scientifique, Orleans, France.

Petit C

Roche A C

Article Info

Journal

Analytical biochemistry

Abbr.

Anal Biochem

ISSN

0003-2697

DOI

10.1016/0003-2697(88)90578-7

Published

1988-12-00

Pages

525-30

Language

English

Region

United States

NLM ID

0370535

Subset

External Links

PubMed source DOI: 10.1016/0003-2697(88)90578-7 View journal details

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Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod.

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