Home LiteratureArticle Details
PMID: 3243272 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

VirD proteins of Agrobacterium tumefaciens are required for the formation of a covalent DNA--protein complex at the 5' terminus of T-strand molecules.

The EMBO journal ·Vol. 7 ·No. 13 ·1988-12-20 ·Pages 4055-62

Herrera-Estrella A, Chen ZM, Van Montagu M, Wang K

Abstract

The T-DNA transfer process of Agrobacterium tumefaciens is activated by the induction of the Ti plasmid virulence (vir) loci by plant signal molecules such as acetosyringone. Upon initiation of the T-DNA transfer process, site-specific nicks occur at the 25-bp border sequences. This cleavage leads to the generation of a free, linear ssT-DNA molecule which is bound by sequence non-specific VirE proteins. Here we present evidence for the involvement of other acetosyringone-induced proteins in the formation of a covalent complex between the T-strand and protein, designated the T-complex. Alkaline gel-electrophoretic analysis showed that proteins specifically bind to the 5' termini of nicked T-DNA molecules. The T-complex can be formed in Escherichia coli when the VirD1 and VirD2 proteins are expressed.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism DNA, Bacterial/metabolism Genes, Bacterial Molecular Sequence Data Plants/metabolism Rhizobium/genetics,metabolism Virulence Factors
Chemicals
Bacterial Proteins DNA, Bacterial T-DNA Virulence Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Herrera-Estrella A
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Chen Z M
Van Montagu M
Wang K
References (48)
48 references, click to expand
  1. Analysis of the complete nucleotide sequence of the Agrobacterium tumefaciens virB operon.
    Nucleic Acids Res. 1988 May 25;16(10):4621-36 PMID: 2837739
  2. Site-Specific Nick in the T-DNA Border Sequence as a Result of Agrobacterium vir Gene Expression.
    Science. 1987 Jan 30;235(4788):587-91 PMID: 17758248
  3. Transgenic plants as tools to study the molecular organization of plant genes.
    Science. 1987 Sep 4;237(4819):1176-83 PMID: 17801640
  4. Isolation and identification of TL-DNA/plant junctions in Convolvulus arvensis transformed by Agrobacterium rhizogenes strain A4.
    EMBO J. 1985 Dec 1;4(12):3069-77 PMID: 16453649
  5. Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping.
    Plasmid. 1980 Mar;3(2):193-211 PMID: 6100893
  6. virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens.
    Cell. 1986 Aug 1;46(3):325-33 PMID: 3731272
  7. Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus.
    Proc Natl Acad Sci U S A. 1986 Apr;83(8):2541-5 PMID: 3085097
  8. Chimeric genes as dominant selectable markers in plant cells.
    EMBO J. 1983;2(6):987-95 PMID: 16453464
  9. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli.
    J Mol Biol. 1980 Apr;138(2):179-207 PMID: 6997493
  10. Transfecting deoxyribonucleic acid of Bacillus bacteriophage phi 29 that is protease sensitive.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1555-9 PMID: 4504368
  11. Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity.
    EMBO J. 1983;2(12):2143-50 PMID: 16453482
  12. Agrobacterium tumefaciens and the susceptible plant cell: a novel adaptation of extracellular recognition and DNA conjugation.
    Cell. 1986 Oct 24;47(2):155-7 PMID: 3768954
  13. Characterization of the virB operon from an Agrobacterium tumefaciens Ti plasmid.
    J Biol Chem. 1988 Apr 25;263(12):5804-14 PMID: 3281947
  14. Relaxation complexes of plasmid DNA and protein. I. Strand-specific association of protein and DNA in the relaxed complexes of plasmids ColE1 and ColE2.
    J Biol Chem. 1975 Nov 25;250(22):8785-9 PMID: 1102543
  15. New cloning vehicles for transformation of higher plants.
    EMBO J. 1985 Feb;4(2):277-84 PMID: 16453603
  16. The genetic and transcriptional organization of the vir region of the A6 Ti plasmid of Agrobacterium tumefaciens.
    EMBO J. 1986 Jul;5(7):1445-54 PMID: 3017694
  17. Identification of a protein linked to the ends of adenovirus DNA.
    Cell. 1977 Jun;11(2):283-95 PMID: 196758
  18. Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9006-10 PMID: 3480525
  19. Molecular characterization of a host-range-determining locus from Agrobacterium tumefaciens.
    J Bacteriol. 1986 Oct;168(1):244-50 PMID: 3759904
  20. DNA Replication of bacteriophage phi29: isolation of a DNA-protein complex from Bacillus subtilis cells infected with wild-type and with a suppressor-sensitive mutant.
    Virology. 1976 Sep;73(2):389-401 PMID: 822582
  21. Structure of nascent phiX174 replicative form: evidence for discontinuous DNA replication.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):984-8 PMID: 4522806
  22. Processing of plasmid DNA during bacterial conjugation.
    Microbiol Rev. 1984 Mar;48(1):24-41 PMID: 6201705
  23. Relaxation complexes of poasmid DNA and protein. III. Association of protein with the 5' terminus of the broken DNA strand in the relaxed complex of plasmid ColE1.
    J Biol Chem. 1975 Nov 25;250(22):8796-803 PMID: 1102545
  24. Characterization of Agrobacterium tumefaciens virulence proteins induced by the plant factor acetosyringone.
    J Mol Biol. 1987 Oct 20;197(4):635-45 PMID: 3430596
  25. Identification and genetic analysis of an Agrobacterium tumefaciens chromosomal virulence region.
    J Bacteriol. 1985 Mar;161(3):850-60 PMID: 2982791
  26. Processing of the T-DNA of Agrobacterium tumefaciens generates border nicks and linear, single-stranded T-DNA.
    J Bacteriol. 1987 Mar;169(3):1046-55 PMID: 3029014
  27. Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2669-73 PMID: 2986128
  28. Genome-linked protein associated with the 5' termini of bacteriophage phi29 DNA.
    J Virol. 1978 Sep;27(3):776-83 PMID: 100608
  29. Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58.
    J Bacteriol. 1987 Nov;169(11):5101-12 PMID: 2822665
  30. Single-stranded DNA binding proteins required for DNA replication.
    Annu Rev Biochem. 1986;55:103-36 PMID: 3527040
  31. Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: requirement for 5' virD gene products.
    EMBO J. 1987 Apr;6(4):857-63 PMID: 3595560
  32. A protein covalently linked to poliovirus genome RNA.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):59-63 PMID: 189316
  33. A plant cell factor induces Agrobacterium tumefaciens vir gene expression.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):379-83 PMID: 16593648
  34. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  35. Bacteriophage phi29 terminal protein: its association with the 5' termini of the phi29 genome.
    J Virol. 1978 Dec;28(3):895-904 PMID: 731797
  36. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  37. T-DNA border sequences required for crown gall tumorigenesis.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5112-6 PMID: 3860847
  38. Identification of the protein firmly bound to the ends of bacteriophage phi 29 DNA.
    Virology. 1978 Feb;84(2):279-92 PMID: 203093
  39. Molecular characterization of the virD operon from Agrobacterium tumefaciens.
    Nucleic Acids Res. 1987 Sep 25;15(18):7503-17 PMID: 3658701
  40. Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from agrobacterium to the plant genome.
    Cell. 1984 Sep;38(2):455-62 PMID: 6467373
  41. Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA molecules in Escherichia coli by a virD-encoded border-specific endonuclease from Agrobacterium tumefaciens.
    J Bacteriol. 1987 Nov;169(11):5035-45 PMID: 2822660
  42. The virD operon of Agrobacterium tumefaciens encodes a site-specific endonuclease.
    Cell. 1986 Nov 7;47(3):471-7 PMID: 3021341
  43. Relaxation complexes of plasmid DNA and protein. II. Characterization of the proteins associated with the unrelaxed and relaxed complexes of plasmid ColE1.
    J Biol Chem. 1975 Nov 25;250(22):8790-5 PMID: 1102544
  44. Short direct repeats flank the T-DNA on a nopaline Ti plasmid.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6322-6 PMID: 16593241
  45. Tumor induction by Agrobacterium tumefaciens: analysis of the boundaries of T-DNA.
    J Mol Appl Genet. 1982;1(4):361-70 PMID: 7108407
  46. Purification of a cellular, double-stranded DNA-binding protein required for initiation of adenovirus DNA replication by using a rapid filter-binding assay.
    Mol Cell Biol. 1986 May;6(5):1363-73 PMID: 3785168
  47. Single-Stranded DNA Binding Protein Encoded by the virE Locus of Agrobacterium tumefaciens.
    Science. 1988 Apr 22;240(4851):501-4 PMID: 17784072
  48. Selection, mapping, and characterization of osmoregulatory mutants of Escherichia coli blocked in the choline-glycine betaine pathway.
    J Bacteriol. 1986 Mar;165(3):856-63 PMID: 3512526
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-12-20
Pages
4055-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC455113
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2