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PMID: 325374 Published · ppublish English Journal Article

Suppression of polarity of insertion mutations within the gal operon of E. coli.

Molecular & general genetics : MGG ·Vol. 151 ·No. 3 ·1977-03-16 ·Pages 295-304

Besemer J, Herpers M

Abstract

Phenotypic revertants of galOP::IS1 and galOP::IS2 mutations have been isolated after mutagenesis with nitrosoguanidine, they are probably caused by mutations in gene suA. The polarity suppressor mutations described in this study and a known mutation in gene suA isolated by D. Morse (Morse and Guertin, 1972) suppress polarity caused by IS1 more effectively than that caused by IS2 or IS4. Furthermore, suppressibility is influenced by the site and orientation of IS integration. The synthesis of the three enzymes in galOP::IS suA double mutants is constitutive and the ratio of the three enzymes is altered in comparison to the wild type. The reasons for constitutive synthesis of the galactose enzymes and for the altered ratio of enzyme synthesis are discussed.

MeSH Terms
DNA, Bacterial Escherichia coli Extrachromosomal Inheritance Galactose/metabolism Mutation Operon Suppression, Genetic
Chemicals
DNA, Bacterial Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Besemer J
Herpers M
References (36)
36 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268793
Published
1977-03-16
Pages
295-304
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268793
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