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PMID: 325526 Published · ppublish English Journal Article

Transcription in vitro of bacteriophage lambda 4S RNA: studies on termination and rho protein.

Nucleic acids research ·Vol. 4 ·No. 4 ·1977-04-00 ·Pages 827-42

Howard BH, de Crombrugghe B, Rosenberg M

Abstract

When bacteriophage lambdapga18 DNA is transcribed in a purified in vitro system by E. coli RNA polymerase (nucleoside triphosphate: RNA nucleotidyl-transferase, EC 2.7.7.6), several major transcripts are synthesized. We have investigated transcriptional termination of one of these transcripts, the 4S, or "oop" RNA. Analysis by two-dimensional "fingerprinting" of T1 oligonucleotides reveals that transcription of the 4S RNA terminates at a specific site on the lambdapga18 DNA template, t-L with an efficiency of approximately 80%, i.e. 20% of transcripts are extended into larger RNAs. Addition of the E. coli protein rho to our transcription reactions has two effects: a) the efficiency of termination at the t-L site is increased to 100%; b) the number of 4S transcripts synthesized is increased by greater than 5-fold. Rho appears to stimulate 4S RNA synthesis by facilitating more rapid release of RNA polymerase from the t-L' termination site.

MeSH Terms
Binding Sites Coliphages/enzymology DNA, Viral/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Operon RNA, Viral/biosynthesis Rho Factor/pharmacology Ribonucleases Species Specificity Templates, Genetic Transcription Factors/pharmacology Transcription, Genetic
Chemicals
DNA, Viral RNA, Viral Rho Factor Transcription Factors DNA-Directed RNA Polymerases Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Howard B H
de Crombrugghe B
Rosenberg M
References (29)
29 references, click to expand
  1. Polarity suppressors defective in transcription termination at the attenuator of the tryptophan operon of Escherichia coli have altered rho factor.
    J Mol Biol. 1976 Sep 15;106(2):231-41 PMID: 135845
  2. Activation of transcription at specific promoters by glycerol.
    J Biol Chem. 1974 Jul 10;249(13):4050-6 PMID: 4368372
  3. Regulation of the expression of the histidine operon in Salmonella typhimurium.
    Nature. 1974 Jun 7;249(457):523-7 PMID: 4599761
  4. Kappa, a novel factor for the arrest of transcription in vitro by DNA-dependent RNA polymerase from Escherichia coli at specific sites of natural templates.
    Eur J Biochem. 1973 Mar 1;33(2):201-6 PMID: 4571592
  5. Effect of Rho on transcription of bacterial operons.
    Nat New Biol. 1973 Feb 28;241(113):260-4 PMID: 4348358
  6. A possible termination factor for transcription in Escherichia coli.
    Biochem Biophys Res Commun. 1974 Feb 4;56(3):725-31 PMID: 4363749
  7. Role of cyclic adenosine 3',5'-monophosphate and the cyclic adenosine 3',5'-monophosphate receptor protein in the initiation of lac transcription.
    J Biol Chem. 1971 Dec 10;246(23):7343-8 PMID: 4333322
  8. In vitro transcription of the gal operon requires cyclic adenosine monophosphate and cyclic adenosine monophosphate receptor protein.
    J Biol Chem. 1971 Aug 10;246(15):4671-8 PMID: 4327324
  9. ATPase activity required for termination of transcription by the Escherichia coli protein factor rho.
    J Biol Chem. 1976 Apr 25;251(8):2520-4 PMID: 131127
  10. Termination of transcription in vitro in the Escherichia coli tryptophan operon leader region.
    J Mol Biol. 1976 May 15;103(2):383-93 PMID: 781272
  11. Regulation of transcription termination in the leader region of the tryptophan operon of Escherichia coli involves tryptophan or its metabolic product.
    J Mol Biol. 1976 May 15;103(2):339-49 PMID: 781270
  12. Dual-control of the tryptophan operon is mediated by both tryptophanyl-tRNA synthetase and the repressor.
    J Mol Biol. 1976 May 15;103(2):209-26 PMID: 781267
  13. Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1959-63 PMID: 132662
  14. Transcription termination and late control in phage lambda.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3300-4 PMID: 1059112
  15. Termination of transcription in bacteriophage lambda. Heterogeneous, 3'-terminal oligo-adenylate additions and the effects of rho factor.
    J Biol Chem. 1975 Jun 25;250(12):4755-64 PMID: 1095576
  16. Thr region between the operator and first structural gene of the tryptophan operon of Escherichia coli may have a regulatory function.
    J Mol Biol. 1973 May 5;76(1):89-101 PMID: 4578102
  17. Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2534-8 PMID: 4601822
  18. Selective effects of MgCl2 and temperature on the initiation of transcription at lac, gal, and lambda promoters.
    J Biol Chem. 1975 Oct 25;250(20):8202-8 PMID: 170282
  19. Control of short leftward transcripts from the immunity and ori regions in induced coliphage lambda.
    Mol Gen Genet. 1973 Nov 22;126(4):275-90 PMID: 4784811
  20. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.
    Eur J Biochem. 1969 Dec;11(2):395-9 PMID: 4902612
  21. Termination factor for RNA synthesis.
    Nature. 1969 Dec 20;224(5225):1168-74 PMID: 4902144
  22. Influence of salts on RNA synthesis by DNA-dependent RNA-polymerase from Escherichia coli.
    Eur J Biochem. 1967 Dec;3(2):183-93 PMID: 4865567
  23. A two-dimensional fractionation procedure for radioactive nucleotides.
    J Mol Biol. 1965 Sep;13(2):373-98 PMID: 5325727
  24. Transcription termination factor rho activity is altered in Escherichia coli with suA gene mutations.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1725-8 PMID: 1098042
  25. Evidence that mutations in the suA polarity suppressing gene directly affect termination factor rho.
    Nature. 1976 Jan 15;259(5539):151-3 PMID: 1107855
  26. Nucleotide sequence of the 5' end of tryptophan messenger RNA of Escherichia coli.
    J Mol Biol. 1976 May 15;103(2):351-81 PMID: 781271
  27. Transcription termination in vivo in the leader region of the tryptophan operon of Escherichia coli.
    J Mol Biol. 1976 May 15;103(2):319-37 PMID: 781269
  28. Determination of nucleotide sequences beyond the sites of transcriptional termination.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):717-21 PMID: 1062781
  29. 4S oop RNA is a leader sequence for the immunity-establishment transcription in coliphage lambda.
    Nature. 1976 Jul 8;262(5564):112-6 PMID: 934330
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.4.827
Published
1977-04-00
Pages
827-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342487
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.4.827 PMC Full Text View journal details
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