Home LiteratureArticle Details
PMID: 325556 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A gene from Escherichia coli affecting the sigma subunit of RNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 5 ·1977-05-00 ·Pages 1836-40

Harris JD, Martinez II, Calendar R

Abstract

The RNA polymerase sigma subunits of Escherichia coli K, E. coli C, and Salmonella typhimurium can be resolved by electrophoresis. Using this technique, we have analyzed Salmonella strains carrying F' plasmids from E. coli K in order to map the gene for the sigma factor. Partial diploid analyses show the location of the sigma gene at 62-66 min on the E. coli genetic map. This gene is cotransducible with toIC and dnaG, at 66 min.

MeSH Terms
Chromosome Mapping DNA, Bacterial/metabolism DNA, Recombinant/metabolism DNA-Directed RNA Polymerases/biosynthesis Electrophoresis, Polyacrylamide Gel Escherichia coli/metabolism F Factor Genes Genetic Linkage Plasmids Salmonella typhimurium/metabolism Sigma Factor/biosynthesis Transcription Factors/biosynthesis
Chemicals
DNA, Bacterial DNA, Recombinant Sigma Factor Transcription Factors DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Harris J D
Martinez I I
Calendar R
References (25)
25 references, click to expand
  1. A bacteriophage specific for F- Salmonella strains.
    Science. 1961 Jun 30;133(3470):2069-70 PMID: 13788574
  2. Infective heredity in bacteria.
    Cold Spring Harb Symp Quant Biol. 1953;18:261-9 PMID: 13168993
  3. A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts.
    Mol Gen Genet. 1976 Oct 18;148(1):93-8 PMID: 792684
  4. Histidine regulatory mutants in Salmonella typhiumium. VI. Dominance studies.
    J Mol Biol. 1968 May 14;33(3):547-57 PMID: 4302595
  5. Escherichia coli K-12 F-prime factors, old and new.
    Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
  6. The selectivity of transcription.
    Annu Rev Biochem. 1974;43(0):721-75 PMID: 4605337
  7. Loss of the sigma activity of RNA polymerase of Bacillus subtilis during sporulation.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1865-9 PMID: 4198276
  8. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  9. Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2743-7 PMID: 1101264
  10. In vivo and in vitro phosphorylation of DNA-dependent RNA polymerase of Escherichia coli by bacteriophage-T7-induced protein kinase.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2506-10 PMID: 1101258
  11. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
  12. Bacteriophage P2 head morphogenesis: cleavage of the major capsid protein.
    Virology. 1973 May;53(1):1-23 PMID: 4574872
  13. Structural interactions between amino acid residues at positions 22 and 211 in the tryptophan synthetase alpha chain of Escherichia coli.
    J Bacteriol. 1974 Feb;117(2):444-8 PMID: 4590468
  14. Analysis of bacteriophage T7 early RNAs and proteins on slab gels.
    J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
  15. Classification and mapping of spontaneous and induced mutations in the histidine operon of Salmonella.
    Adv Genet. 1971;16:1-34 PMID: 4947105
  16. Purification and physical characterization of tyrosyl ribonucleic acid synthetases from Escherichia coli and Bacillus subtilis.
    Biochemistry. 1966 May;5(5):1681-90 PMID: 4960133
  17. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants.
    J Mol Biol. 1968 Jul 14;34(2):331-46 PMID: 4938550
  18. Localized mutagenesis of any specific small region of the bacterial chromosome.
    Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158-62 PMID: 4943557
  19. Attachment of prophage P2: gene order at different host chromosomal sites.
    Virology. 1969 Dec;39(4):867-81 PMID: 4902260
  20. Control of template specificity of E. coli RNA polymerase by a phage-coded protein.
    Nature. 1969 Sep 13;223(5211):1111-3 PMID: 4897557
  21. A mutation increasing the amount of a constitutive enzyme in Escherichia coli, glucose 6-phosphate dehydrogenase.
    J Mol Biol. 1971 Feb 28;56(1):183-94 PMID: 4929885
  22. Cohesive sites on the deoxyribonucleic acids from several temperate coliphages.
    J Mol Biol. 1966 Jun;17(2):343-57 PMID: 5336321
  23. Factor stimulating transcription by RNA polymerase.
    Nature. 1969 Jan 4;221(5175):43-6 PMID: 4882047
  24. Escherichia coli deoxyribonucleic acid synthesis mutants: their effect upon bacteriophage P2 and satellite bacteriophage P4 deoxyribonucleic acid synthesis.
    J Bacteriol. 1975 Oct;124(1):167-75 PMID: 1100599
  25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.5.1836
Published
1977-05-00
Pages
1836-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431020
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.5.1836 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2