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PMID: 3260006 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Reliability of biotinylated DNA probes in colony hybridization: evaluation of an improved colony lysis method for detection of TEM-1 beta-lactamase.

Molecular and cellular probes ·Vol. 2 ·No. 1 ·1988-03-00 ·Pages 83-5

Huovinen S, Huovinen P, Jacoby GA

Abstract

Utilizing an improved method for colony hybridization developed by Haas & Fleming, biotin and 32P-labelled TEM-1 probes were compared for sensitivity and specificity in identifying the type of beta-lactamase made by over 100 clinical bacterial isolates. The new procedure was more reliable than a standard one, but still gave more than 20% false positive and false negative reactions.

MeSH Terms

Bacteria/classification,enzymology Bacterial Proteins/analysis,genetics Biotin DNA, Recombinant Drug Resistance, Microbial Isoelectric Focusing Nucleic Acid Hybridization Predictive Value of Tests beta-Lactamases/analysis,genetics

Chemicals

Bacterial Proteins DNA, Recombinant Biotin beta-Lactamases beta-lactamase TEM-1

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Huovinen S

Massachusetts General Hospital, Boston 02114.

Huovinen P

Jacoby G A

Article Info

Journal

Molecular and cellular probes

Abbr.

Mol Cell Probes

ISSN

0890-8508

DOI

10.1016/0890-8508(88)90047-3

Published

1988-03-00

Pages

83-5

Language

English

Region

England

NLM ID

8709751

Subset

Grants

FIC NIH HHS · FO5 TWO3785 · United States

NIAID NIH HHS · R01 AI20415 · United States

External Links

PubMed source DOI: 10.1016/0890-8508(88)90047-3 View journal details

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Reliability of biotinylated DNA probes in colony hybridization: evaluation of an improved colony lysis method for detection of TEM-1 beta-lactamase.

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