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PMID: 3261240 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

EGF-stimulated tyrosine phosphorylation of p185neu: a potential model for receptor interactions.

The EMBO journal ·Vol. 7 ·No. 4 ·1988-04-00 ·Pages 995-1001

Stern DF, Kamps MP

Abstract

p185neu is a receptor-like protein encoded by the neu/erbB-2 proto-oncogene. This protein is closely related to the epidermal growth factor (EGF) receptor, but does not bind EGF. We report here that incubation of Rat-1 cells with EGF stimulates tyrosine phosphorylation of p185. This effect is specific to EGF since neither platelet derived growth factor (PDGF) nor insulin, which also bind to receptors with ligand-stimulated tyrosine kinase activity, induced tyrosine phosphorylation of p185. The EGF-stimulated tyrosine phosphorylation of p185 and of the EGF receptor occurred with similar kinetics and EGF dose-responses, and both phosphorylations were prevented by down-regulation of the EGF receptor with EGF. Since p185 does not bind EGF, these results suggested that p185 is a substrate for the EGF receptor kinase. Incubation of cells with EGF before lysis stimulated the tyrosine phosphorylation of p185 in immune complexes. This suggested that EGF, acting through the EGF receptor, can regulate the intrinsic kinase activity of p185.

MeSH Terms
Amino Acids/analysis Animals Antibodies, Monoclonal Cell Line Epidermal Growth Factor/pharmacology ErbB Receptors/physiology Genes Models, Biological Phosphorylation Protein-Tyrosine Kinases/metabolism Proto-Oncogenes Tyrosine
Chemicals
Amino Acids Antibodies, Monoclonal Tyrosine Epidermal Growth Factor ErbB Receptors Protein-Tyrosine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stern D F
Department of Pathology, Yale University School of Medicine, New Haven, CT 06510.
Kamps M P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-04-00
Pages
995-1001
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454426
Subset
IM
Grants
NCI NIH HHS · CA14195 · United States
NCI NIH HHS · CA17289 · United States
NCI NIH HHS · T32CA09370 · United States
External Links
PubMed source PMC Full Text View journal details
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