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PMID: 3261871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 18 ·1988-09-00 ·Pages 6924-8

Trapani JA, Klein JL, White PC, Dupont B

Abstract

A cDNA clone encoding a human serine esterase gene was isolated from a library constructed from poly(A)+ RNA of allogeneically stimulated, interleukin 2-expanded peripheral blood mononuclear cells. The clone, designated HSE26.1, represents a full-length copy of a 0.9-kilobase mRNA present in human cytotoxic cells but absent from a wide variety of noncytotoxic cell lines. Clone HSE26.1 contains an 892-base-pair sequence, including a single 741-base-pair open reading frame encoding a putative 247-residue polypeptide. The first 20 amino acids of the polypeptide form a leader sequence. The mature protein is predicted to have an unglycosylated Mr of approximately equal to 26,000 and contains a single potential site for N-linked glycosylation. The nucleotide and predicted amino acid sequences of clone HSE26.1 are homologous with all murine and human serine esterases cloned thus far but are most similar to mouse granzyme B (70% nucleotide and 68% amino acid identity). HSE26.1 protein is expressed weakly in unstimulated peripheral blood mononuclear cells but is strongly induced within 6-hr incubation in medium containing phytohemagglutinin. The data suggest that the protein encoded by HSE26.1 plays a role in cell-mediated cytotoxicity.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cytotoxicity, Immunologic DNA/analysis Esterases/genetics Humans Molecular Sequence Data RNA Splicing RNA, Messenger/metabolism T-Lymphocytes, Cytotoxic/enzymology
Chemicals
RNA, Messenger DNA Esterases serine esterase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Trapani J A
Laboratory of Human Immunogenetics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Klein J L
White P C
Dupont B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.18.6924
Published
1988-09-00
Pages
6924-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282091
Subset
IM
Grants
NCI NIH HHS · CA 08748 · United States
NCI NIH HHS · CA 22507 · United States
Databases
GENBANK
J04071
External Links
PubMed source DOI: 10.1073/pnas.85.18.6924 PMC Full Text View journal details
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