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PMID: 3264152 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Citrobacter diversus ULA-27 beta-lactamases. Improved purification and general properties.

The Biochemical journal ·Vol. 254 ·No. 3 ·1988-09-15 ·Pages 885-90

Amicosante G, Oratore A, Franceschini N, Maccarrone M, Strom R, Galleni M, Frère JM

Abstract

Two chromosome-encoded beta-lactamases have been purified from Citrobacter diversus ULA-27. They exhibited slightly different isoelectric points (6.8 and 6.2) and very similar Mr values (congruent to 29,000). Their specificity spectrum was rather wide, since they hydrolysed some cephalosporins with kcat: values similar to those observed with the best penicillin substrates. Cloxacillin, methicillin and imipenem were hydrolysed very slowly. Hydrolysis of azthreonam could not be detected.

MeSH Terms
Amino Acids/analysis Cephalosporins/metabolism Chromatography, Affinity Chromosomes, Bacterial/enzymology Citrobacter/enzymology Electrophoresis, Polyacrylamide Gel Isoelectric Point Isoenzymes/isolation & purification Kinetics Molecular Weight Penicillins/metabolism Substrate Specificity beta-Lactamases/isolation & purification
Chemicals
Amino Acids Cephalosporins Isoenzymes Penicillins beta-Lactamases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Amicosante G
Università degli Studi dell'Aquila, Dipartimento di Scienze e Tecnologie Biomediche e di Biometria, Italy.
Oratore A
Franceschini N
Maccarrone M
Strom R
Galleni M
Frère J M
References (15)
15 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2540885
Published
1988-09-15
Pages
885-90
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135165
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2540885 PMC Full Text View journal details
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