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PMID: 3264318 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Loss of CD4 membrane expression and CD4 mRNA during acute human immunodeficiency virus replication.

The Journal of experimental medicine ·Vol. 168 ·No. 6 ·1988-12-01 ·Pages 1953-69

Salmon P, Olivier R, Riviere Y, Brisson E, Gluckman JC, Kieny MP, Montagnier L, Klatzmann D

Abstract

Using mAbs and genomic probe to the CD4 molecule, the HIV receptor, we demonstrated that HIV replication induces the disappearance of its functional receptor from the cell surface by two distinct mechanisms. First, after being expressed onto the cell surface, HIV envelope gp110 will complex CD4, efficiently masking the CD4 epitope used by the virus to bind its receptor. This phenomenon occurs on the surface of each infected cell and is not due to the release of soluble gp110; infection with recombinant HIV/vaccinia viruses expressing a mutated HIV env gene designed to prevent gp110 release from the cell surface induces a similar gp/CD4 complexes formation. Second, virus replication induces a dramatic and rapid loss of CD4 mRNA transcripts, preventing new CD4 molecules from being synthesized. These two mechanisms of receptor modulation could have been developed to avoid reinfection of cells replicating the virus as well as to produce more infectious particles. These results suggest that the classical virus interference documented for other retroviruses might not only be due to receptor/envelope interaction, but might also depend on receptor gene expression.

MeSH Terms
Cell Membrane/metabolism Cells, Cultured Gene Expression Regulation HIV/genetics,growth & development HIV Antigens/metabolism RNA, Messenger/metabolism Receptors, HIV Receptors, Virus/genetics,metabolism Recombination, Genetic Vaccinia virus/genetics Viral Envelope Proteins/metabolism Virus Replication
Chemicals
HIV Antigens RNA, Messenger Receptors, HIV Receptors, Virus Viral Envelope Proteins gp 110 env protein, Human immunodeficiency virus
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Salmon P
Laboratoire de Biologie et Génétique des Déficits Immunitaires, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
Olivier R
Riviere Y
Brisson E
Gluckman J C
Kieny M P
Montagnier L
Klatzmann D
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.168.6.1953
Published
1988-12-01
Pages
1953-69
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189155
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.168.6.1953 PMC Full Text View journal details
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