Cell size in aging monolayer cultures.
Changes in the size of the area covered by individual cultured WI-38 cells as the cultures age have been studied by using a new microphotographic paper cutout technique. This method is nondestructive and noninstrusive and avoids a number of artifacts which can occur in the measurement of suspended cells. The measurements reveal that the decreased cell yield of late passage cultures reflects not only the appearance of a subpopulation of larger cells but also the failure of the cells to utilize all the growth surface available to them.
19 references, click to expand
-
Effect of trypsin on cell volume and mass.Nat New Biol. 1972 Sep 13;239(89):53-5 PMID: 4507214
-
The division cycle and RNA-synthesis in diploid human cells at different passage levels in vitro.Exp Cell Res. 1966 Jun;42(3):673-84 PMID: 5922078
-
The influence of culture medium volume on cell density and lifespan of human diploid fibroblasts.Exp Cell Res. 1975 Mar 15;91(2):389-92 PMID: 1126392
-
Analogy in growth between late passage human embryonic and early passage human adult fibroblasts.J Cell Biol. 1969 Nov;43(2):374-7 PMID: 5344156
-
Aging of human fibroblasts in vitro. Correlations between DNA synthetic ability and cell size.Exp Cell Res. 1975 Jun;93(1):184-90 PMID: 166851
-
Growth limitation of 3T3 mouse fibroblasts by available growth surface area and medium components.J Cell Physiol. 1975 Oct;86(2 Pt 1):301-9 PMID: 1194367
-
Conditions limiting multiplication of fibroblastic and epithelial cells in dense cultures.Nature. 1973 Nov 23;246(5430):197-9 PMID: 4586792
-
The use of frequency distributions of cell diameters to characterize cell populations in tissue culture.Exp Cell Res. 1967 Feb;45(2):336-50 PMID: 6021926
-
The biology of aging.N Engl J Med. 1971 Nov;285(20):1120-9 PMID: 4937862
-
Genealogies of clones of diploid fibroblasts. Cinemicrophotographic observations of cell division patterns in relation to population age.Exp Cell Res. 1974 Sep;88(1):95-104 PMID: 4417691
-
Clonal selection, attenuation and differentiation in an in vitro model of hyperplasia.Am J Pathol. 1974 Jan;74(1):137-54 PMID: 4809311
-
Unique techniques for cell analysis utilizing mithramycin and flow microfluorometry.Exp Cell Res. 1975 Jun;93(1):235-9 PMID: 49273
-
Cell size and nucleic acid content in the diploid human cell line WI-38 during aging.Med Exp Int J Exp Med. 1969;19(6):313-20 PMID: 5408801
-
Cellular senescence and DNA synthesis. Thymidine incorporation as a measure of population age in human diploid cells.Exp Cell Res. 1973 Feb;76(2):419-27 PMID: 4568162
-
The use of trypsin for the determination of cellular viability.Exp Cell Res. 1965 Oct;40(1):186-8 PMID: 5891332
-
Measurement of DNA content and cell volume in senescent human fibroblasts utilizing flow multiparameter single cell analysis.Exp Cell Res. 1976 Mar 15;98(2):298-302 PMID: 1253848
-
Relationship between cell replication and volume in senescent human diploid fibroblasts.Mech Ageing Dev. 1976 Jan-Feb;5(1):45-56 PMID: 1263608
-
Production and characterization of multiple-layered populations of animal cells.J Cell Biol. 1965 Nov;27(2):273-9 PMID: 5884626
-
Enhancement of senescence in low passage human embryonic lung cells by an agent extracted from phase 3 cells.Exp Cell Res. 1973 Apr;79(1):143-51 PMID: 4360527
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