Home LiteratureArticle Details
PMID: 3275631 Published · ppublish English Journal Article

Flagellin domain that affects H antigenicity of Escherichia coli K-12.

Journal of bacteriology ·Vol. 170 ·No. 1 ·1988-01-00 ·Pages 485-8

Kuwajima G

Abstract

Escherichia coli K-12 mutants with altered flagellum antigenicity were isolated by introducing random deletions into the flagellin gene. The deletions were identified in the central region of the gene. It is suggested that this region corresponds to the flagellin domain molecule which affects flagellum antigenicity.

MeSH Terms
Amino Acid Sequence Animals Antigens, Bacterial/genetics,immunology Bacterial Proteins/genetics Base Sequence Cell Movement Chromosome Deletion DNA, Bacterial/genetics Escherichia coli/genetics,immunology Flagella/immunology Flagellin/genetics,immunology Molecular Sequence Data Mutation Rabbits
Chemicals
Antigens, Bacterial Bacterial Proteins DNA, Bacterial H antigen Flagellin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kuwajima G
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
References (19)
19 references, click to expand
  1. Genetics and chemistry of bacterial flagella.
    Bacteriol Rev. 1969 Dec;33(4):454-75 PMID: 4906131
  2. Cleavage of bacterial flagellin with cyanogen bromide. Chemical and physical properties of the protein fragments.
    Biochem J. 1969 Jul;113(3):489-99 PMID: 5807209
  3. Identification of amino acid changes in serological mutants of the i flagellar antigen of Salmonella typhimurium.
    Microbios. 1973;7(25):71-3 PMID: 4572647
  4. Amino acid sequence of flagellin of Bacillus subtilis 168. III. Tryptic peptides, N-bromosuccinimide peptides, and the complete amino acid sequence.
    J Biol Chem. 1976 Feb 10;251(3):705-11 PMID: 814121
  5. Deletion and amber mutants of fla loci in Escherichia coli K-12.
    Genetics. 1976 Nov;84(3):403-21 PMID: 793926
  6. Identification of an antibody binding site in the phase-1 flagellar protein of Salmonella typhimurium.
    Microbios. 1976;15(61-62):221-8 PMID: 796628
  7. Genetics of structure and function of bacterial flagella.
    Annu Rev Genet. 1977;11:161-82 PMID: 339815
  8. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  9. The nucleotide sequence of the Mr = 28,500 flagellin gene of Caulobacter crescentus.
    J Biol Chem. 1983 Jun 25;258(12):7395-401 PMID: 6305939
  10. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  11. Genetic analysis of H2, the structural gene for phase-2 flagellin in Salmonella.
    J Gen Microbiol. 1984 Feb;130(2):255-65 PMID: 6374019
  12. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  13. The covalent structure of the phase-1 flagellar filament protein of Salmonella typhimurium and its comparison with other flagellins.
    J Biol Chem. 1985 Dec 15;260(29):15758-61 PMID: 2999134
  14. Covalent structure of three phase-1 flagellar filament proteins of Salmonella.
    J Mol Biol. 1985 Dec 20;186(4):791-803 PMID: 4093985
  15. Nucleotide sequence of the hag gene encoding flagellin of Escherichia coli.
    J Bacteriol. 1986 Dec;168(3):1479-83 PMID: 3536885
  16. Polymerization of Salmonella flagellin in the presence of high concentrations of salts.
    Biochim Biophys Acta. 1969 Feb 4;175(1):195-203 PMID: 5766001
  17. Deoxyribonucleic acid nucleases. II. The effects of metals on the mechanism of action of deoxyribonuclease I.
    J Biol Chem. 1968 Sep 10;243(17):4409-16 PMID: 5683998
  18. Regions of Salmonella typhimurium flagellin essential for its polymerization and excretion.
    J Bacteriol. 1987 Jan;169(1):291-6 PMID: 3539920
  19. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.1.485-488.1988
Published
1988-01-00
Pages
485-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210675
Subset
IM
Databases
GENBANK
M19037
External Links
PubMed source DOI: 10.1128/jb.170.1.485-488.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2