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PMID: 3277174 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Uptake of oleate by isolated rat adipocytes is mediated by a 40-kDa plasma membrane fatty acid binding protein closely related to that in liver and gut.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 2 ·1988-01-00 ·Pages 359-63

Schwieterman W, Sorrentino D, Potter BJ, Rand J, Kiang CL, Stump D, Berk PD

Abstract

A portion of the hepatocellular uptake of nonesterified long-chain fatty acids is mediated by a specific 40-kDa plasma membrane fatty acid binding protein, which has also been isolated from the gut. To investigate whether a similar transport process exists in other tissues with high transmembrane fatty acid fluxes, initial rates (Vo) of [3H]oleate uptake into isolated rat adipocytes were studied as a function of the concentration of unbound [3H]oleate in the medium. Vo reached a maximum as the concentration of unbound oleate was increased (Km = 0.30 +/- 0.03 microM; Vmax = 2470 +/- 90 pmol/min per 5 X 10(4) adipocytes) and was significantly inhibited both by phloretin and by prior incubation of the cells with Pronase. A rabbit antibody to the rat liver plasma membrane fatty acid binding protein inhibited adipocyte fatty acid uptake by up to 63% in dose-dependent fashion. Inhibition was noncompetitive; at an immunoglobulin concentration of 250 micrograms/ml Vmax was reduced from 2480 +/- 160 to 1870 +/- 80 pmol/min per 5 X 10(4) adipocytes, with no change in Km. A basic (pI approximately equal to 9.1) 40-kDa adipocyte plasma membrane fatty acid binding protein, isolated from crude adipocyte plasma membrane fractions, reacted strongly in both agar gel diffusion and electrophoretic blots with the antibody raised against the corresponding hepatic plasma membrane protein. These data indicate that the uptake of oleate by rat adipocytes is mediated by a 40-kDa plasma membrane fatty acid binding protein closely related to that in liver and gut.

MeSH Terms
Adipose Tissue/cytology,metabolism Animals Carrier Proteins/metabolism Cell Membrane/metabolism Fatty Acid-Binding Protein 7 Fatty Acid-Binding Proteins Fluorescent Antibody Technique In Vitro Techniques Intestinal Mucosa/metabolism Kinetics Liver/metabolism Male Molecular Weight Neoplasm Proteins Nerve Tissue Proteins Oleic Acid Oleic Acids/metabolism Organ Specificity Rats Rats, Inbred Strains
Chemicals
Carrier Proteins Fabp7 protein, rat Fatty Acid-Binding Protein 7 Fatty Acid-Binding Proteins Neoplasm Proteins Nerve Tissue Proteins Oleic Acids Oleic Acid
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Schwieterman W
Department of Medicine, Mount Sinai School of Medicine, City University of New York, NY 10029.
Sorrentino D
Potter B J
Rand J
Kiang C L
Stump D
Berk P D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.2.359
Published
1988-01-00
Pages
359-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279547
Subset
IM
Grants
NIAAA NIH HHS · AA-06860 · United States
NIADDK NIH HHS · AM-26438 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.2.359 PMC Full Text View journal details
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