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PMID: 3278689 Published · ppublish English Journal Article

Nucleotide sequence and expression of the Enterobacter aerogenes alpha-acetolactate decarboxylase gene in brewer's yeast.

Applied and environmental microbiology ·Vol. 54 ·No. 1 ·1988-01-00 ·Pages 38-42

Sone H, Fujii T, Kondo K, Shimizu F, Tanaka J, Inoue T

Abstract

The nucleotide sequence of a 1.4-kilobase DNA fragment containing the alpha-acetolactate decarboxylase gene of Enterobacter aerogenes was determined. The sequence contains an entire protein-coding region of 780 nucleotides which encodes an alpha-acetolactate decarboxylase of 260 amino acids. The DNA sequence coding for alpha-acetolactate decarboxylase was placed under the control of the alcohol dehydrogenase I promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both S. cerevisiae and Escherichia coli. Brewer's yeast cells transformed by this plasmid showed alpha-acetolactate decarboxylase activity and were used in laboratory-scale fermentation experiments. These experiments revealed that the diacetyl concentration in wort fermented by the plasmid-containing yeast strain was significantly lower than that in wort fermented by the parental strain. These results indicated that the alpha-acetolactate decarboxylase activity produced by brewer's yeast cells degraded alpha-acetolactate and that this degradation caused a decrease in diacetyl production.

MeSH Terms
Base Sequence Carboxy-Lyases/genetics DNA, Bacterial/analysis Enterobacter/enzymology,genetics Enterobacteriaceae/enzymology Fermentation Genes, Bacterial Lactates/metabolism Molecular Sequence Data Plasmids Saccharomyces cerevisiae/enzymology Transformation, Genetic
Chemicals
DNA, Bacterial Lactates alpha-acetolactate Carboxy-Lyases acetolactate decarboxylase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sone H
Central Laboratories of Key Technology, Kirin Brewery Co., Ltd., Gunma, Japan.
Fujii T
Kondo K
Shimizu F
Tanaka J
Inoue T
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.54.1.38-42.1988
Published
1988-01-00
Pages
38-42
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC202393
Subset
IM
Databases
GENBANK
J03433
External Links
PubMed source DOI: 10.1128/aem.54.1.38-42.1988 PMC Full Text View journal details
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